SureClick™ Antibody Labeling Kit

Covalent, Fc-targeted antibody conjugation in just two hours

Invitrogen SureClick Antibody Labeling Kits enable fast, precise, and reproducible conjugation of human or rabbit IgG antibodies using trusted click chemistry. Designed for spatial biology researchers and biotherapeutic developers, SureClick kits attach an average of 2 payloads per antibody at the Fc region, preserving antigen binding and Fc receptor (FcR) affinity.

SureClick is an exceptional solution for attaching oligonucleotides to antibodies, making it ideal for spatial biology workflows, immuno-PCR, and next-generation antibody-based detection platforms that require precise and reproducible antibody–oligo conjugation.

Achieve consistent, covalent antibody labeling with a simplified workflow amenable to automation.

What is SureClick antibody labeling?

SureClick is a site-specific antibody conjugation technology that covalently attaches payloads—including fluorophores, functional tags, and oligonucleotides—to the Fc region of IgG antibodies using click chemistry. Unlike conventional random labeling approaches, SureClick:

• Targets the Fc domain
• Preserves Fab antigen-binding regions
• Maintains Fc glycosylation integrity
• Enables reproducible degree of labeling (DOL)
• Supports consistent antibody–oligonucleotide conjugation

Key features and benefits:

Precise, reproducible labeling

• Site-specific Fc conjugation
• Consistent attachment of ~2 payloads per antibody
• Reduced heterogeneity compared to conventional methods

Rapid two-hour workflow

• ~10 minutes hands-on time
• 90-minute protocol
• Simple protocol using a single Fc-targeting component

Covalent and stable conjugation

• Trusted click chemistry
• Robust linkage for downstream assays, spatial biology, and antibody–oligo workflows

Preserves antibody function

• Confidence that antigen binding is unchanged
• Maintains Fc receptor interactions

Automation-ready

• Spin-column purification
• Minimal components
• Suitable for high-throughput workflows

How SureClick antibody labeling works

1. Add SureClick Fc-targeting reagent to your IgG antibody
2. Purify using included Zeba™ spin columns
3. Add click chemistry payload of choice (e.g., fluorophores, oligonucleotides, functional tags)

The result is a site-specific, covalently labeled antibody with consistent performance, including highly controlled antibody–oligonucleotide conjugates.

Applications

• Spatial biology
• Antibody–oligonucleotide conjugation
• Multiplex biomarker detection
• Antibody-drug conjugate (ADC) development
• Biotherapeutic characterization
• Flow cytometry
• Internalization studies
• Biomarker validation