iBind™ Cards
iBind™ Cards
Invitrogen™

iBind™ Cards

iBind Cards are used with the iBind Western Device, which is an automated western-processing device that performs every step in자세히 알아보기
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카탈로그 번호수량
SLF1010X440 cards
SLF101010 cards
카탈로그 번호 SLF1010X4
제품 가격(KRW)
865,000
40 cards
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수량:
40 cards
제품 가격(KRW)
865,000
40 cards
카트에 추가하기
iBind Cards are used with the iBind Western Device, which is an automated western-processing device that performs every step in the western workflow from blocking to washes to antibody incubations. The iBind Western Device performs the steps via sequential lateral flow (SLF). SLF allows the timely release and flow of solutions and antibodies to the membrane without need of an external power source, shakers, or trays.

Learn more about the iBind western systems >

iBind Card features include:
• Glass-fiber matrix allowing optimal and homogenous flow of immunodetection solutions
• Long shelf life—store iBind Cards for 12 months at room temperature
• Better antigen-antibody interaction—solutions flow between the iBind Card and membrane for maximum interaction between protein and antibody

The iBind Cards, together with the iBind Western Device and iBind Solution Kit or iBind Fluorescent Detection (FD) Solution Kit, bring automation and simplicity to your western protocol. Each iBind Card can run one mini-membrane, and each box contains 10 cards. An iBind Western Device is required for use of the cards.
For Research Use Only. Not for use in diagnostic procedures.
사양
수량40 cards
배송 조건Room Temperature
용량1 Mini Blot/Card
검출 방법Chromogenic, Chemiluminescence, Fluorescence
용도(애플리케이션)Western Blot
용도 (장비)iBind Western Device
Membrane CompatibilityNitrocellulose, PVDF
제품라인iBind
처리량1 Mini Blot/Card
유형Card
Unit Size40 cards
구성 및 보관
Store at room temperature.

자주 묻는 질문(FAQ)

What procedure should I follow when stripping and reprobing my blot processed using the iBind Western Device or iBind Flex Western Device?

To strip and reprobe your blot processed using the iBind Western Device or iBind Flex Western Device, first strip the blot outside the system using your preferred protocol. After the stripping protocol, wash the blot in distilled water for 5 min. Reblocking is unnecessary as the iBind Solution will perform this step during reprobing. Finally, reprobe with new antibodies using the iBind Western Device or iBind Flex Western Device, starting with the membrane preparation step.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I multiplex antibodies using the iBind Western Device or iBind Flex Western Device?

Yes, for each midi blot, mini blot, or vertically cut strip, prepare appropriate primary antibodies together in a single tube of iBind FD/ iBind Flex FD Solution, Prepare appropriate secondary antibodies together in a different tube of iBind FD/ iBind Flex FD Solution. Do not mix primary and secondary antibodies prior to loading in the device. All primary and secondary antibodies should be diluted according to the recommendations outlined in the user manual or quick reference card. When detecting several targets of the same species, the same concentration of secondary antibody can be used as when detecting one target. There is no need to increase the concentration for each additional target. Load multiplexed antibodies into the device as normal.
Caution: Consider cross-reactivity of secondary antibodies when multiplexing (e.g., rabbit anti-goat IgG and goat anti-mouse IgG are likely to cross-react).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the iBind Western Device or iBind Flex Western Device with Alexa Fluor Plus conjugated secondary antibodies?

Yes, both the iBind Western Device and iBind Flex Western Device work well with Alexa Fluor Plus conjugated secondary antibodies.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

With the iBind Western Device and iBind Flex Western Device, how do I know if I am rolling the membrane firmly enough?

When firmly rolling, the iBind/iBind Flex Card will slightly dip in the membrane area, causing the top and bottom of the card to lift. See the iBind/iBind Flex user manual for additional details.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Is there any difference between the iBind Flex Card and the original iBind Card?

Besides being ~1.7 times as wide, the iBind Flex Card also includes alignment lines printed on the back that appear when solution is added to the card to help in proper horizontal placement of multiple mini or vertically cut strip blots.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

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