Galacton-Star Substrate is a chemiluminescent substrate designed for the rapid, sensitive detection of β-galactosidase in cell lysates. The chemiluminescent assay exhibits over three orders of magnitude greater sensitivity compared to colorimetric β-galactosidase assays. Light emission from Galacton-Star Substrate reaches a maximum in 60-90 minutes and remains constant for at least 1 hour. After incubation at room temperature, the signal is measured in a luminometer.

As few as 10 femtograms of β-galactosidase (20,000 molecules) are detectable. High sensitivity makes this substrate ideal for detection of weak expression and for transfection normalization with other sensitive reporter gene assays.