Tetramethylrhodamine, Ethyl Ester, Perchlorate (TMRE)

인용 및 참조 문헌 (159)

Invitrogen™

Tetramethylrhodamine, Ethyl Ester, Perchlorate (TMRE)

Tetramethylrhodamine, ethyl ester (TMRE) is a cell-permeant, cationic, red-orange fluorescent dye that is readily sequestered by active mitochondria.Dissolve the dye자세히 알아보기

카탈로그 번호	수량
T669	25 mg

카탈로그 번호 T669

제품 가격(KRW)

357,000

Online offer

Ends: 31-Dec-2025

396,000

할인액 39,000 (10%)

25 mg

카트에 추가하기

25 mg

제품 가격(KRW)

357,000

Online offer

Ends: 31-Dec-2025

396,000

할인액 39,000 (10%)

25 mg

카트에 추가하기

Tetramethylrhodamine, ethyl ester (TMRE) is a cell-permeant, cationic, red-orange fluorescent dye that is readily sequestered by active mitochondria.

Dissolve the dye in high-quality anhydrous DMSO or ethanol to prepare stock concentration at 1 to 10 mM.

Visualize staining your cell without wasting your reagents, antibodies, or time with our new Stain-iT Cell Staining Simulator.

For Research Use Only. Not for use in diagnostic procedures.

검출 방법Fluorescence

여기/방출549/574 nm

분자량514.96

수량25 mg

배송 조건Room Temperature

세포하위구조 국지화Mitochondria

색상Red-Orange

용도 (장비)Fluorescence Microscope

제품 유형TMRE

Unit Size25 mg

구성 및 보관

Store in freezer (-5°C to -30°C) and protect from light.

자주 묻는 질문(FAQ)

I am seeing high background outside of my neuronal cells when using membrane potential indicators. What can I do to reduce background?

If you use our FluoVolt Membrane Potential Kit (Cat. No. F10488), the kit provides a background suppressor to reduce this problem. For other indicators, consider the use of BackDrop Background Suppressor (Cat no. R37603, B10511, and B10512).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between fast and slow-response membrane potential probes?

Molecules that change their structure in response to the surrounding electric field can function as fast-response probes for the detection of transient (millisecond) potential changes. Slow-response dyes function by entering depolarized cells and binding to proteins or membranes. Increased depolarization results in additional dye influx and an increase in fluorescence, while hyperpolarization is indicated by a decrease in fluorescence. Fast-response probes are commonly used to image electrical activity from intact heart tissues or measure membrane potential changes in response to pharmacological stimuli. Slow-responding probes are often used to explore mitochondrial function and cell viability.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What type of membrane potential indicators do you offer and how should I choose one for my experiment?

A membrane potential indicator selection guide can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-viability-and-regulation/ion-indicators/membrane-potential-indicators.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (159)

인용 및 참조 문헌

Abstract

A hyperfused mitochondrial state achieved at G1-S regulates cyclin E buildup and entry into S phase.

Authors:Mitra K, Wunder C, Roysam B, Lin G, Lippincott-Schwartz J,

Journal:Proc Natl Acad Sci U S A

PubMed ID:19617534

Mitochondria undergo fission-fusion events that render these organelles highly dynamic in cells. We report a relationship between mitochondrial form and cell cycle control at the G(1)-S boundary. Mitochondria convert from isolated, fragmented elements into a hyperfused, giant network at G(1)-S transition. The network is electrically continuous and has greater ATP ... More

Antiapoptotic effect of nicorandil mediated by mitochondrial atp-sensitive potassium channels in cultured cardiac myocytes.

Authors:Akao M, Teshima Y, MarbÃ¡n E

Journal:J Am Coll Cardiol

PubMed ID:12204514

OBJECTIVES: We examined whether nicorandil, a clinically useful drug for the treatment of ischemic syndromes, inhibits myocardial apoptosis. BACKGROUND: Nicorandil has been reported to have a cardioprotective action through activation of mitochondrial ATP-sensitive potassium (mitoK(ATP)) channels. Based on our recent observation that mitoK(ATP) channel activation has a remarkable antiapoptotic effect ... More

Synaptic mitochondria are more susceptible to Ca2+overload than nonsynaptic mitochondria.

Authors:Brown MR, Sullivan PG, Geddes JW

Journal:J Biol Chem

PubMed ID:16517608

'Mitochondria in nerve terminals are subjected to extensive Ca2+ fluxes and high energy demands, but the extent to which the synaptic mitochondria buffer Ca2+ is unclear. In this study, we identified a difference in the Ca2+ clearance ability of nonsynaptic versus synaptic mitochondrial populations enriched from rat cerebral cortex. Mitochondria ... More

Age-related macular degeneration. The lipofusion component N-retinyl-N-retinylidene ethanolamine detaches proapoptotic proteins from mitochondria and induces apoptosis in mammalian retinal pigment epithelial cells.

Authors:Suter M, Remé C, Grimm C, Wenzel A, Jäättela M, Esser P, Kociok N, Leist M, Richter C

Journal:J Biol Chem

PubMed ID:11006290

'10-20% of individuals over the age of 65 suffer from age-related macular degeneration (AMD), the leading cause of severe visual impairment in humans living in developed countries. The pathogenesis of this complex disease is poorly understood, and no efficient therapy or prevention exists to date. A precondition for AMD appears ... More

VDAC-dependent permeabilization of the outer mitochondrial membrane by superoxide induces rapid and massive cytochrome c release.

Authors:Madesh M, Hajnóczky G

Journal:J Cell Biol

PubMed ID:11739410

'Enhanced formation of reactive oxygen species (ROS), superoxide (O2*-), and hydrogen peroxide (H2O2) may result in either apoptosis or other forms of cell death. Here, we studied the mechanisms underlying activation of the apoptotic machinery by ROS. Exposure of permeabilized HepG2 cells to O2*- elicited rapid and massive cytochrome c ... More

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