Mitochondrial Membrane Potential Apoptosis Kit, with Mitotracker™ Red & Annexin V Alexa Fluor™ 488, for flow cytometry

인용 및 참조 문헌 (4)

Invitrogen™

Mitochondrial Membrane Potential Apoptosis Kit, with Mitotracker™ Red & Annexin V Alexa Fluor™ 488, for flow cytometry

This flow cytometry product provides a rapid and convenient assay for two hallmarks of apoptosis - phosphatidylserine externalization and changes자세히 알아보기

카탈로그 번호	수량
V35116	1 kit

카탈로그 번호 V35116

제품 가격(KRW)

670,000

온라인 행사

Ends: 31-Dec-2025

788,000

할인액 118,000 (15%)

1 kit

카트에 추가하기

1 kit

제품 가격(KRW)

670,000

온라인 행사

Ends: 31-Dec-2025

788,000

할인액 118,000 (15%)

1 kit

카트에 추가하기

This flow cytometry product provides a rapid and convenient assay for two hallmarks of apoptosis - phosphatidylserine externalization and changes in mitochondrial membrane potential. After staining a cell population with Alexa Fluor™ 488 annexin V and MitoTracker™ Red CMXRos dye in the provided binding buffer, live cells exhibit very little green fluorescence and bright red fluorescence, whereas apoptotic cells exhibit green fluorescence and decreased red fluorescence. These populations can easily be distinguished using a flow cytometer, and the 488 nm line of an argon-ion laser can be used to excite both dyes.

View a selection guide for all apoptosis assays for flow cytometry.

For Research Use Only. Not for use in diagnostic procedures.

여기/방출Mitotracker™ red 579⁄599, Alexa Fluor™ 488: 499⁄521

유세포분석기 레이저 라인488

용도(애플리케이션)Flow Cytometry

용도(장비)Fluorescence Microscope, Flow Cytometer

반응 수50

제품라인Alexa Fluor, MitoTracker

제품 유형Mitochondrial Membrane Apoptosis Kit

수량1 kit

배송 조건Wet Ice

콘주게이트Alexa Fluor 488, MitoTracker Red

형식Tube, Slide

Unit Size1 kit

구성 및 보관

Contains 1 vial of annexin, Alexa Fluor™ 488 conjugate (250 μL), 3 vials of MitoTracker™ red (50 μg per vial), 1 bottle of annexin binding buffer (5X solution, 15 mL), and 1 vial of DMSO (100 μL).

Store in refrigerator (2–8°C) and protect from light.

자주 묻는 질문(FAQ)

What are the fluorescence excitation/emission maxima for the dyes contained in the Mitochondrial Membrane Potential Apoptosis Kit, with Mitotracker Red & Alexa Fluor 488 annexin V, for flow cytometry

Alexa Fluor 488 annexin V: 499/521 nm
MitoTracker Red: 579/599 nm

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to study apoptosis using an Annexin V conjugate, but with adherent cells via microscopy instead of flow cytometry. Can this be done?

It has been done, but we don‘t recommend it. Both healthy cells and apoptotic cells possess phosphatidylserine on the cell surface, which can be detected with Annexin V, but apoptotic cells have significantly more of it. You can easily tell the difference between these two populations with flow cytometry, because flow cytometers are more sensitive and have a higher throughput. But with a microscope, you cannot always tell the difference, especially for adherent cells. Instead, for microscopy, we recommend a different technique, such as detecting caspases with CellEvent Caspase Detection Reagents.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (4)

인용 및 참조 문헌

Abstract

Galectin 1 modulates plasma cell homeostasis and regulates the humoral immune response.

Authors:Anginot A, Espeli M, Chasson L, Mancini SJ, Schiff C,

Journal:J Immunol

PubMed ID:23616571

'Galectin-1 (GAL1) is an S-type lectin with multiple functions, including the control of B cell homeostasis. GAL1 expression was reported to be under the control of the plasma cell master regulator BLIMP-1. GAL1 was detected at the protein level in LPS-stimulated B cells and was shown to promote Ig secretion ... More

FGF23 activates injury-primed renal fibroblasts via FGFR4-dependent signalling and enhancement of TGF-ß autoinduction.

Authors:Smith ER, Holt SG, Hewitson TD

Journal:Int J Biochem Cell Biol

PubMed ID:28919046

Bone-derived fibroblast growth factor 23 (FGF23) is an important endocrine regulator of mineral homeostasis with effects transduced by cognate FGF receptor (FGFR)1-a-Klotho complexes. Circulating FGF23 levels rise precipitously in patients with kidney disease and portend worse renal and cardiovascular outcomes. De novo expression of FGF23 has been found in the ... More

High Tidal Volume Induces Mitochondria Damage and Releases Mitochondrial DNA to Aggravate the Ventilator-Induced Lung Injury.

Authors:Lin JY, Jing R, Lin F, Ge WY, Dai HJ, Pan L

Journal:Front Immunol

PubMed ID:30018615

This study aimed to determine whether high tidal volume (HTV) induce mitochondria damage and mitophagy, contributing to the release of mitochondrial DNA (mtDNA). Another aim of the present study was to investigate the role and mechanism of mtDNA in ventilator-induced lung injury (VILI) in rats. ... More

Adaptive phenotypic modulations lead to therapy resistance in chronic myeloid leukemia cells.

Authors:Baykal-Köse S, Acikgoz E, Yavuz AS, Gönül Geyik Ö, Ates H, Sezerman OU, Özsan GH, Yüce Z

Journal:PLoS One

PubMed ID:32106243

Tyrosine kinase inhibitor (TKI) resistance is a major problem in chronic myeloid leukemia (CML). We generated a TKI-resistant K562 sub-population, K562-IR, under selective imatinib-mesylate pressure. K562-IR cells are CD34-/CD38-, BCR-Abl-independent, proliferate slowly, highly adherent and form intact tumor spheroids. Loss of CD45 and other hematopoietic markers reveal these cells have ... More