Our pTrcHis A, B, & C vectors are designed to offer enhanced translation initiation and high-level expression in E. coli. These vectors feature:
• High-level regulated transcription from the trc promoter • Enhanced translation efficiency of eukaryotic genes in E. coli • The lacO operator and lacIq repressor gene for transcriptional regulation in any E. coli strain
This particular vector offers:
• C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an anti-His(C-term) antibody • C-terminal c-myc epitope for easy detection of fusion proteins with an anti-myc antibody
For Research Use Only. Not for use in diagnostic procedures.
사양
구성 또는 유도성 시스템
Inducible
유도제
IPTG
프로모터
Trc, lacO
제품 유형
Bacterial Expression Vector
선택 제제(진핵)
None
항생제 내성 박테리아
Ampicillin (AmpR)
분열
EK (Enterokinase) Recognition Site
단백질 태그
Thioredoxin
클로닝 방법
Restriction Enzyme/MCS
벡터
pTrc
구성 및 보관
All vectors are supercoiled and are provided lyophilized. A TOP10 E. coli stab and positive expression control are provided. Store vectors at -20°C. Store stab at room temperature. The vector is guaranteed stable for 6 months when properly stored.