pLenti6.2/V5-DEST™ Gateway™ Vector
본 제품은 LMO 제품으로, 고객 분께서 LMO 신고 시스템을 통해 직접 수입 신고를 진행해주셔야 합니다. 자세히보기
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Invitrogen™

pLenti6.2/V5-DEST™ Gateway™ Vector

The pLenti6.2⁄V5-DEST™ Gateway™ Vector is a Gateway™-adapted ViraPower™ II lentiviral expression vector for lentiviral-based expression of a target gene in자세히 알아보기
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카탈로그 번호수량
V368206 μg
카탈로그 번호 V36820
제품 가격(KRW)
2,601,000
Online offer
Ends: 31-Mar-2026
3,060,000
할인액 459,000 (15%)
6 µg
카트에 추가하기
수량:
6 μg
제품 가격(KRW)
2,601,000
Online offer
Ends: 31-Mar-2026
3,060,000
할인액 459,000 (15%)
6 µg
카트에 추가하기
The pLenti6.2⁄V5-DEST™ Gateway™ Vector is a Gateway™-adapted ViraPower™ II lentiviral expression vector for lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The vector has the CMV promoter for driving constitutive expression of the target gene and the PGK promoter for driving long-term, persistent expression of the Blasticidin stable selection marker.

Advantages
• Stable expression
• Long-term experiments
• High-throughput screening
• Accurate titer of functional virus (using Blasticidin method)
• Flexible and versatile Gateway™ recombination cloning technology

Key Features
• Human cytomegalovirus (CMV) immediate early promoter to control high-level expression of the gene of interest
• PKG Promoter for expression of Blasticidin selection marker

Kit includes
• pLenti6.2⁄V5-DEST™ Gateway™ Vector box
• One Shot™ Stbl3™ chemically competent E. coli (C7373-03)

Related SKUs
• 293FT Cell Line (R70007; R70007)
• ViraPower™ Lentiviral Support Kit (K4970-00)
• ViraPower™ Lentiviral Packaging Mix (K4975-00)
• Lipofectamine™ 2000 (11668-019; 11668-027)

For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
사양
구성 또는 유도성 시스템Constitutive
배달 유형Lentiviral
용도(애플리케이션)Viral Expression
제품 유형Lentiviral Expression Vector
수량6 μg
선택 제제(진핵)Blasticidin
선택 마커 프로모터PGK Promoter
벡터pLenti
클로닝 방법Gateway
제품라인Gateway, ViraPower
프로모터CMV
단백질 태그V5 Epitope Tag
Unit Size6 µg
구성 및 보관
pLenti6.2⁄V5-DEST™ Gateway™ Vector Box contains pLenti6.2⁄V5-DEST, 40 μl of 150 ng⁄μl vector in 10 mM Tris-HCl, 1mM EDTA, pH 8.0 and pLenti6.2⁄V5-GW⁄lacZ control, 20 μl of 0.5 μg⁄μl vector in 10 mM Tris-HCl, 1mM EDTA, pH 8.0. Store at –20°C.

One Shot™ Stbl3™ chemically competent E. coli box contains transformation reagents including 20 single-use 50 μl aliquots of One Shot™ Competent Cells, S.O.C. medium, and pUC19 supercoiled plasmid control. Store at -80°C.

자주 묻는 질문(FAQ)

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.