The pBAD/gIII Kit provides all of the necessary reagents to express recombinant proteins under the tight regulation of the araBAD promoter. To simplify purification, the vector pBAD/gIII encodes a leader peptide which directs the recombinant protein into the periplasmic space. The vector provides:
• The araBAD promoter for tightly regulated expression • Translation initiation signals for optimized E. coliexpression • Leader peptide from the bacteriophage fd gene III protein (gIII) for secreted expression • C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin • C-terminal c-myc epitope for detection and analysis with an Anti-myc Antibody
A set of three vectors is provided (A, B, and C). Each has the C-terminal tag in a different reading frame relative to the multiple cloning site to simplify in-frame cloning of your gene.
For Research Use Only. Not for use in diagnostic procedures.
사양
구성 또는 유도성 시스템
Inducible
유도제
Arabinose
프로모터
araBAD
제품 유형
Bacterial Expression Vector
선택 제제(진핵)
None
항생제 내성 박테리아
Ampicillin (AmpR)
단백질 태그
c-Myc Epitope Tag
클로닝 방법
Restriction Enzyme/MCS
벡터
pBAD
구성 및 보관
The pBAD/gIII Kit includes 20 μg each of pBAD/gIII A, B, & C, 20 μg of pBAD/gIII/calmodulin, 1 ml 20% L-arabinose, LMG194 and TOP10 E. coli stabs. Store stabs at 2 - 8°C. Store all other components at -20°C. All components are guaranteed stable for 6 months when properly stored.