pAd/BLOCK-iT™-DEST RNAi Gateway Vector
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인용 및 참조 문헌 (2)
Invitrogen™

pAd/BLOCK-iT™-DEST RNAi Gateway Vector

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast,자세히 알아보기
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카탈로그 번호수량
V49220
V492-20으로도 사용됨
6 μg
카탈로그 번호 V49220
V492-20으로도 사용됨
제품 가격(KRW)
1,705,000
Online offer
Ends: 31-Mar-2026
1,894,000
할인액 189,000 (10%)
6 µg
카트에 추가하기
수량:
6 μg
제품 가격(KRW)
1,705,000
Online offer
Ends: 31-Mar-2026
1,894,000
할인액 189,000 (10%)
6 µg
카트에 추가하기
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway™ destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available.

Additional materials required, available separately: Gateway™ entry clone, Gateway™ LR Clonase™ enzyme mix, and reaction buffer.
For Research Use Only. Not for use in diagnostic procedures.
사양
구성 또는 유도성 시스템Constitutive
배달 유형Adenoviral
제품 유형RNAi Gateway Vector
수량6 μg
벡터pAd, pDEST
클로닝 방법Gateway
제품라인BLOCK-iT, Gateway
프로모터U6
Unit Size6 µg
구성 및 보관
All destination vectors are provided lyophilized and supercoiled.

자주 묻는 질문(FAQ)

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all pAd/BLOCK-iT™-DEST RNAi Gateway Vector FAQs

인용 및 참조 문헌 (2)

인용 및 참조 문헌
Abstract
Thymidine phosphorylase is angiogenic and promotes tumor growth.
Authors:Moghaddam A, Zhang HT, Fan TP, Hu DE, Lees VC, Turley H, Fox SB, Gatter KC, Harris AL, Bicknell R
Journal:Proc Natl Acad Sci U S A
PubMed ID:7532308
'Platelet-derived endothelial cell growth factor was previously identified as the sole angiogenic activity present in platelets; it is now known to be thymidine phosphorylase (TP). The effect of TP on [methyl-3H]thymidine uptake does not arise from de novo DNA synthesis and the molecule is not a growth factor. Despite this, ... More
Gene silencing in alveolar type II cells using cell-specific promoter in vitro and in vivo.
Authors:Gou D, Narasaraju T, Chintagari NR, Jin N, Wang P, Liu L,
Journal:Nucleic Acids Res
PubMed ID:15452203
RNA interference (RNAi) is a sequence-specific post-transcriptional gene silencing process. Although it is widely used in the loss-of-function studies, none of the current RNAi technologies can achieve cell-specific gene silencing. The lack of cell specificity limits its usage in vivo. Here, we report a cell-specific RNAi system using an alveolar ... More