pAd/CMV/V5-DEST™ Gateway™ Vector Kit
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인용 및 참조 문헌 (4)
Invitrogen™

pAd/CMV/V5-DEST™ Gateway™ Vector Kit

pAd⁄CMV⁄V5-DEST™ Gateway™ Vector Kit에는 분열(dividing) 및 미분열(non dividing) 포유류 세포에서 표적 유전자의 재조합 기반 클로닝과 adenoviral 기반 일시적 발현을 쉽게자세히 알아보기
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카탈로그 번호수량
V49320
V493-20으로도 사용됨
6 μg
카탈로그 번호 V49320
V493-20으로도 사용됨
제품 가격(KRW)
1,825,000
Online offer
Ends: 31-Mar-2026
2,085,000
할인액 260,000 (12%)
6 µg
카트에 추가하기
수량:
6 μg
제품 가격(KRW)
1,825,000
Online offer
Ends: 31-Mar-2026
2,085,000
할인액 260,000 (12%)
6 µg
카트에 추가하기
pAd⁄CMV⁄V5-DEST™ Gateway™ Vector Kit에는 분열(dividing) 및 미분열(non dividing) 포유류 세포에서 표적 유전자의 재조합 기반 클로닝과 adenoviral 기반 일시적 발현을 쉽게 할 수 있는 Gateway™-adapted ViraPower™ adenoviral 발현 벡터, pAd⁄CMV⁄V5-DEST™ 벡터가 들어있습니다. 이 벡터는 CMV promoter로 높은 수준의 구성요소 발현이 유도되는 표적 유전자를 가진 adenovirus을 생성할 수 있습니다.

장점
• 높은 efficiency 및 빠른 재조합 클로닝
• 높은 역가의 adenoviral stock 생성
• in vitro 또는 in vivo에서 유전자를 분할 및 미분할 포유류 세포로 효율적으로 이동
• 복제 불가 바이러스를 생성하여 시스템의 생물안전성을 높입니다.
• high-throughput 어플리케이션에 맞게 수정 가능

핵심 특징
• 효율적이고 빠른 클로닝을 위한 Gateway™ 기술
• 관심 유전자에서 높은 수준의 구성요소 발현을 나타내도록 하는 CMV promoter
• 발현 패키지에 대한 Human Ad5 sequences (ΔE3) 및 Viral Inverted Terminal Repeats (ITRs)가 virion 구성
• mRNA의 효율적인 transcription termination 및 polyadenylation을 위한 단순 헤르페스 바이러스 thymidine kinase (TK) polyadenylation 서열
• 재조합 단백질을 검출하는 V5 epitope
• Ampicillin 선택 표지자

Kit 구성
• pAd⁄CMV⁄V5-DEST™ Gateway™ Vector
• pAd⁄CMV⁄V5-GW⁄lacZ control plasmid

연구용으로만 사용할 수 있습니다. 치료 또는 진단 목적으로 사용할 수 없습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
구성 또는 유도성 시스템Constitutive
배달 유형Adenoviral
용도(애플리케이션)Viral Expression
제품 유형Mammalian Expression Vector
수량6 μg
벡터pDEST
클로닝 방법Gateway
제품라인Gateway, ViraPower
프로모터CMV
단백질 태그V5 Epitope Tag
Unit Size6 µg
구성 및 보관
• pAd-CMV⁄V5-DEST™ Vector 150 ng⁄μl in TEBuffer, pH 8.0. 40 μl (Store at -20°C)
• pAd⁄CMV⁄V5-GW⁄lacZ control plasmid 1 μg⁄μl in TE Buffer, pH 8.0. 10 μl (Store at -20°C)

자주 묻는 질문(FAQ)

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all pAd/CMV/V5-DEST™ Gateway™ Vector Kit FAQs

인용 및 참조 문헌 (4)

인용 및 참조 문헌
Abstract
Disruption of glucose sensing and insulin secretion by ribozyme Kir6.2-gene targeting in insulin-secreting cells.
Authors:Li L, Rojas A, Wu J, Jiang C,
Journal:Endocrinology
PubMed ID:15166124
'The ATP-sensitive K+ (KATP) channel, composed of Kir6.2 and sulfonylurea receptor (SUR1), in pancreatic beta-cells is believed to serve as a metabolic sensor regulating insulin secretion according to glucose levels. Thus, genetic disruption of Kir6.2 expression may impair KATP channel function in glucose sensing and insulin secretion. Here we show ... More
The receptor for parathyroid hormone and parathyroid hormone-related peptide is hydrolyzed and its signaling properties are altered by directly binding the calpain small subunit.
Authors:Shimada M, Mahon MJ, Greer PA, Segre GV,
Journal:Endocrinology
PubMed ID:15691895
'We show calcium-dependent, direct binding between the N-terminal portion of the PTH/PTHrP receptor (PTH1R) C-terminal intracellular tail and the calpain small subunit. Binding requires, but may not be limited to, amino acids W474, S475, and W477. The wild-type, full-length rat (r) PTH1R, but not rPTH1R with W474A/W477A substitutions, copurifies with ... More
Estrogen related receptors stimulate pyruvate dehydrogenase kinase isoform 4 (PDK4) gene expression.
Authors:Zhang Y, Ma K, Sadana P, Chowdhury F, Gaillard S, Wang F, McDonnell DP, Unterman TG, Elam MB, Park EA,
Journal:J Biol Chem
PubMed ID:17079227
The pyruvate dehydrogenase complex (PDC) catalyzes the conversion of pyruvate to acetyl-CoA in mitochondria and is a key regulatory enzyme in the oxidation of glucose to acetyl-CoA. Phosphorylation of PDC by the pyruvate dehydrogenase kinases (PDK2 and PDK4) inhibits PDC activity. Expression of the PDK genes is elevated in diabetes ... More
Adipose-specific expression, phosphorylation of Ser794 in insulin receptor substrate-1, and activation in diabetic animals of salt-inducible kinase-2.
Authors:Horike N, Takemori H, Katoh Y, Doi J, Min L, Asano T, Sun XJ, Yamamoto H, Kasayama S, Muraoka M, Nonaka Y, Okamoto M,
Journal:J Biol Chem
PubMed ID:12624099
Salt-inducible kinase (SIK), first cloned from the adrenal glands of rats fed a high salt diet, is a serine/threonine protein kinase belonging to an AMP-activated protein kinase family. Induced in Y1 cells at an early stage of ACTH stimulation, it regulated the initial steps of steroidogenesis. Here we report the ... More