pLenti6.3/V5-DEST™ Gateway™ Vector Kit
본 제품은 LMO 제품으로, 고객 분께서 LMO 신고 시스템을 통해 직접 수입 신고를 진행해주셔야 합니다. 자세히보기
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Invitrogen™

pLenti6.3/V5-DEST™ Gateway™ Vector Kit

Invitrogen™ 's pLenti6.3⁄V5-DEST™ Gateway™ Vector Kit is part of our ViraPower™ HiPerform™ Lentiviral Gateway™ Expression Kit (catalog K5330-00). The pLenti6.3⁄V5-DEST™자세히 알아보기
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카탈로그 번호수량
V53306
V533-06으로도 사용됨
1 kit
카탈로그 번호 V53306
V533-06으로도 사용됨
제품 가격(KRW)
3,108,000
Online offer
Ends: 31-Mar-2026
3,656,000
할인액 548,000 (15%)
1 kit
카트에 추가하기
수량:
1 kit
제품 가격(KRW)
3,108,000
Online offer
Ends: 31-Mar-2026
3,656,000
할인액 548,000 (15%)
1 kit
카트에 추가하기
Invitrogen™ 's pLenti6.3⁄V5-DEST™ Gateway™ Vector Kit is part of our ViraPower™ HiPerform™ Lentiviral Gateway™ Expression Kit (catalog K5330-00).

The pLenti6.3⁄V5-DEST™ Gateway™ Vector Kit contains the Gateway™-adapted ViraPower™ HiPerform™ lentiviral expression vector, pLenti6.3⁄V5-DEST™ for easy recombination-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.

Advantages
• Stable expression
• Long-term experiments
• Accurate titer of functional virus
• Flexible and versatile Gateway™ recombination cloning technology

Key Features
• HiPerform™ WPRE and cPPT elements
• CMV promoter
• V5 epitope tag at C terminus
• Blasticidin selection

Kit includes
• A lacZ vector as a positive control, pLenti6.3⁄V5-GW⁄lacZ
• Stbl3™ competent cells

For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
사양
구성 또는 유도성 시스템Constitutive
배달 유형Lentiviral
용도(애플리케이션)Viral Expression
제품 유형Kit, Vector
수량1 kit
선택 제제(진핵)Blasticidin
벡터pDEST, pLenti
클로닝 방법Gateway
제품라인Gateway, HiPerform, ViraPower
프로모터CMV
Unit Size1 kit
구성 및 보관
Vector Kit (Box 1)
pLenti6.3⁄V5-DEST™ (150 ng⁄μL): 6 μg, store at -20°C
pLenti6.3⁄V5-GW⁄lacZ Control vector (500 ng⁄μL): 10 μg, store at -20°C

One Shot™ Stbl3™ Chemically Competent E. coli (Box 2)
Stbl3™ Cells: 21 x 50 μL, store at -80°C
pUC19 control DNA (10 pg⁄μL): 50 μL, store at -80°C
S.O.C. Medium: 6 mL, store at -80°C

자주 묻는 질문(FAQ)

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.