Varioskan™ ALF Multimode Microplate Reader
Varioskan™ ALF Multimode Microplate Reader
Varioskan™ ALF Multimode Microplate Reader
Varioskan™ ALF Multimode Microplate Reader
Varioskan™ ALF Multimode Microplate Reader
Varioskan™ ALF Multimode Microplate Reader
Varioskan™ ALF Multimode Microplate Reader
Thermo Scientific™

Varioskan™ ALF Multimode Microplate Reader

The Thermo Scientific Varioskan ALF Multimode Microplate Reader is a versatile, entry level reader for most common absorbance, turbidimetric, fluorescence, and luminescence microplate assays.
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카탈로그 번호워런티
VA000010C1-year Standard
카탈로그 번호 VA000010C
제품 가격(KRW)
26,000,000
1 each
카트에 추가하기
워런티:
1-year Standard
제품 가격(KRW)
26,000,000
1 each
카트에 추가하기
The Thermo Scientific Varioskan ALF Multimode Microplate Reader is a versatile, entry level reader for most common absorbance, turbidimetric, fluorescence, and luminescence microplate assays. Its photometric optical design is based on a xenon flash lamp and monochromator, enabling continuous wavelength selection from UV through visible range up to 1000 nm. The broad wavelength range enables many common assays, including nucleic acid and protein quantification, bacterial growth curves, ELISA, and cell viability.

Enhance your fluorescence and luminescence measurements with the filter-based optics

With the Varioskan ALF reader, fluorescence and luminescence measurements are performed with filter-based optics for optimal specificity and sensitivity. The system comes with three pre-installed excitation and emission filter sets suitable for many common fluorometric applications like Quant-IT DNA quantitation assays and PrestoBlue and AlamarBlue cell viability assays. Additionally, five open filter positions are available for customized fluorometric and luminometric measurements. The filter slots are easy to access on the instrument's side with no tools required for filter exchange. Also, commonly used FRET and BRET assays are easy to set up and perform thanks to Varioskan ALF reader's unique filter-based optics design.

This package includes a Varioskan ALF Multimode Microplate Reader (Cat. No. VA000010C) and a Rapid Exchange two-year extended warranty.

Achieve consistently accurate results with automatic gain adjustment

The automatic gain adjustment feature selects the ideal fluorometric or luminometric reading range for your instrument based on signal intensity in the well, eliminating the need to manually adjust measurement parameters. The results are consistent no matter what signals are measured.

Additional features

  • Kinetic and endpoint measurements for a variety of applications
  • Spectral scanning for photometric measurements
  • Separate optimized measurement modes for absorbance and turbidimetric measurements
  • A self-diagnostics system that includes a set of initialization tests and adjustments, ensuring instrument readiness for operation
  • Compatible with 6- to 384-well microplates with and without lids
  • Reads μDrop and μDrop Duo plates for microvolume analysis of DNA, RNA, and proteins
  • Performs onboard shaking and incubation for temperature-critical assays
  • Operated through SkanIt software
    • Unlimited installation on multiple computers, no annual fee required
    • Includes an extensive cloud-based library of ready-made protocols
    • Two editions available: Research Edition for life sciences and Drug Discovery Edition with features for compliance with 21 CFR Part 11
    • Multiple user interface languages available
    • Automation compatibility for integration with automation interface
  • Thermo Scientific verification plates provide comprehensive and traceable validation of the instrument optical performance. Ready-made SkanIt software protocols provide easy to read reports with defined measurement parameter limits

Varioskan ALF Multimode Microplate Reader with 2-year Extended Warranty (A66711), REX is a Varioskan ALF specific package SKU including the instrument (VA000010C), and an extended warranty to 2 years which includes a rapid exchange service (REX) should the instrument get broken during the warranty period.

For Research Use Only. Not for use in diagnostic procedures.
사양
흡수 범위200 to 1000 nm
정확도(광도)±1.0% + 0.003 Abs (0 to 2 Abs), ±2.0% (2.0 to 2.5 Abs) at 450 nm (96-well plate)
대역폭Photometry: 2.5 nm
깊이(미터법)61 cm
치수(길이 x 폭 x 높이)61 x 34 x 40 cm
동적 범위Fluorometry and Luminometry: ≥6 Decades
방출 파장 범위210-720 nm
여기 파장 범위200-710 nm
필터Three factory installed fluorometric filter pairs: Coumarin family (Excitation 345/25 nm, Emission 450/40 nm); Fluorescein family (Excitation 485/15 nm, Emission 525/15 nm); Resorufin family (Excitation 555/15 nm, Emission 615/45 nm)
용도(애플리케이션)Photometric, fluorometric and luminometric microplate assays
높이(미터법)40 cm
포함SkanIt software, Research Edition
선형성0 to 2.5 Abs, 2.0% at 450 nm (96-well plate)
측정 속도Photometry: ≤10 sec. (fast mode); Fluorometry and Luminometry: ≤20 sec. (96-well plate)
정밀도SD <0.003 Abs, or CV <1.0% at 450 nm
제품 유형Multimode Microplate Reader
수량Microplate Reader + 1 year warranty
민감도Photometry (DNA): <1 ng/μL (96-well half area plate); Turbidimetry (formazin): <5 turbidity units (FTUs) (96-well plate); Fluorometry (fluorescein): 0.35 fmol/well (black 384-well plate); Luminometry (ATP): <275 amol/well (white 96-well plate)
소프트웨어 언어 버전English, French, German, Spanish, Portuguese, Italian, Japanese, Chinese
스펙트럼 스캔 속도≤16 sec. (Fast mode)
전압100/115/220/230 V
워런티1-year Standard
중량(미터법)24.5 kg
폭(미터법)34 cm
큐벳No
검출기 유형Photomultiplier Tube (PMT)
인큐베이터 온도Ambient +2° to 45°C
인터페이스PC Software (Thermo Scientific SkanIt Software)
광원Xenon Flash Lamp
제품라인Varioskan
Reading OptionsEndpoint, Kinetic
쉐이킹Linear, Orbital, Double Orbital
Software TypeSkanIt Software
Unit Size1 each

자주 묻는 질문(FAQ)

Can relative fluorescence units (RFUs) be compared between two different plate readers?

Relative fluorescence units (RFUs) cannot be compared between two different microplate readers. RFU values are arbitrary and dimensionless, meaning that the actual numbers don't have a specific physical meaning, but the relative values are used for comparison. The critical measurement is the ratio of signal to background.

Whether the signal is low or high is 100% dependent on the baseline, i.e., if the sample signal is 2 and the baseline is 1, then it is a low signal but if the sample signal is 2 and the baseline is 0.01, then it is a very high signal. Instruments that typically give sample signals in the tens of thousands RFUs also typically have baseline RFUs somewhere in the tens or hundreds. For example, the Varioskan Microplate Reader is designed such that the baseline is kept below 0.1 RFU.