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인용 및 참조 문헌 (4)
Invitrogen™
ZOOM™ IPGRunner™ Mini-cell
The ZOOM™ IPGRunner™ System is the first system to offer oil-free, trouble-free 2D electrophoresis in a mini-gel format. First dimension자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| ZM0001 | 1 system |
카탈로그 번호 ZM0001
제품 가격(KRW)
-
수량:
1 system
The ZOOM™ IPGRunner™ System is the first system to offer oil-free, trouble-free 2D electrophoresis in a mini-gel format. First dimension separation isoelectric focusing (IEF) is complete in less than three hours. The mini-gel design of the system is easy to handle, eliminates mineral oil overlays, and allows processing of up to 12 samples at once for high-throughput usage.
The ZOOM™ IPGRunner™ System consists of three main components:
• The ZOOM™ IPGRunner™ Mini-Cell. (Figure 1) includes a high-voltage electrophoresis core and lid assembly that fits a mini-cell chamber identical to the XCell SureLock™ mini-cell. The mini-cell holds two ZOOM™ IPGRunner™ Cassettes.
• The ZOOM™ IPGRunner™ Cassettes (Figure 2) are provided ready to use and self-contained, eliminating oil overlays for convenient sample rehydration and IEF. Each cassette holds up to six ZOOM™ Strips.
• The ZOOM™ Strips are 7 cm long and contain a thin layer of polyacrylamide gel that includes a fixed pH gradient. Each ZOOM™ Strip is clearly labeled with a unique identifying number, pH range, and orientation marks (Figure 3). ZOOM™ Strips are supplied attached to a tri-fold card (Figure 4) for easy access and removal.
Quick and Simple Set-up Procedure
Figure 5 demonstrates how easy and fast it is to load samples and set up the ZOOM™ IPGRunner™ System for IEF. Once IEF is complete, the ZOOM™ IPGRunner™ Mini-Cell can be used with NuPAGE™ and Novex™ ZOOM™ gels to complete SDS-PAGE analysis in 40 minutes.
The ZOOM™ IPGRunner™ System consists of three main components:
• The ZOOM™ IPGRunner™ Mini-Cell. (Figure 1) includes a high-voltage electrophoresis core and lid assembly that fits a mini-cell chamber identical to the XCell SureLock™ mini-cell. The mini-cell holds two ZOOM™ IPGRunner™ Cassettes.
• The ZOOM™ IPGRunner™ Cassettes (Figure 2) are provided ready to use and self-contained, eliminating oil overlays for convenient sample rehydration and IEF. Each cassette holds up to six ZOOM™ Strips.
• The ZOOM™ Strips are 7 cm long and contain a thin layer of polyacrylamide gel that includes a fixed pH gradient. Each ZOOM™ Strip is clearly labeled with a unique identifying number, pH range, and orientation marks (Figure 3). ZOOM™ Strips are supplied attached to a tri-fold card (Figure 4) for easy access and removal.
Quick and Simple Set-up Procedure
Figure 5 demonstrates how easy and fast it is to load samples and set up the ZOOM™ IPGRunner™ System for IEF. Once IEF is complete, the ZOOM™ IPGRunner™ Mini-Cell can be used with NuPAGE™ and Novex™ ZOOM™ gels to complete SDS-PAGE analysis in 40 minutes.
For Research Use Only. Not for use in diagnostic procedures.
사양
수량1 system
용도 (장비)XCell SureLock™ Mini, ZOOM™ IPGRunner™ Mini
Gel CompatibilityZOOM™ IPG Strips
젤 크기7 cm Strips
제품라인IPGRunner, ZOOM
Unit Size1 system
구성 및 보관
The ZOOM IPGRunner Mini-Cell includes the ZOOM IPGRunner Buffer Core and Lid, Gel Tension Wedge, Buffer Dam, and the Mini- Cell chamber.
자주 묻는 질문(FAQ)
What is the protocol for using ZOOM gels after first-dimension separation on an IPG strip?
After the first dimension separation using the ZOOM IPGRunner, I do not see any distinct spots. What could the issue be?
I just set up the ZOOM IPGRunner for IEF but don't see any current running through the system. What is the issue?
What are the power supply requirements for the ZOOM IPGRunner?
What is the tolerance of the ZOOM IPGRunner Mini-Cell to organic solvents?
인용 및 참조 문헌 (4)
인용 및 참조 문헌
Abstract
Myeloid-related protein-14 is a p38 MAPK substrate in human neutrophils.
Journal:J Immunol
PubMed ID:15905572
The targets of the p38 MAPK pathway that mediate neutrophil functional responses are largely unknown. To identify p38 MAPK targets, a proteomic approach was applied in which recombinant active p38 MAPK and [(32)P]ATP were added to lysates from unstimulated human neutrophils. Proteins were separated by two-dimensional gel electrophoresis, and phosphoproteins
Epstein-Barr virus protein kinase BGLF4 is a virion tegument protein that dissociates from virions in a phosphorylation-dependent process and phosphorylates the viral immediate-early protein BZLF1.
Journal:J Virol
PubMed ID:16698993
Epstein-Barr virus (EBV) BGLF4 is a viral protein kinase that is expressed in the lytic phase of infection and is packaged in virions. We report here that BGLF4 is a tegument protein that dissociates from the virion in a phosphorylation-dependent process. We also present evidence that BGLF4 interacts with and
Akt-mediated valosin-containing protein 97 phosphorylation regulates its association with ubiquitinated proteins.
Journal:J Biol Chem
PubMed ID:16027165
Hypoxia is a common environmental stress that influences signaling pathways and cell function. Previous studies from our laboratory have identified significant differences in cellular responses to sustained or intermittent hypoxia with the latter proving more cytotoxic. We hypothesized that differences in susceptibility of neurons to intermittent (IH) and sustained hypoxia
Hsp27 regulates Akt activation and polymorphonuclear leukocyte apoptosis by scaffolding MK2 to Akt signal complex.
Journal:J Biol Chem
PubMed ID:17510053
We have shown previously that Akt exists in a signal complex with p38 MAPK, MAPK-activated protein kinase-2 (MK2), and heat shock protein 27 (Hsp27) and MK2 phosphorylates Akt on Ser-473. Additionally, dissociation of Hsp27 from Akt, prior to Akt activation, induced polymorphonuclear leukocyte (PMN) apoptosis. However, the role of Hsp27