Novex™ Zymogram Plus (Gelatin) Protein Gels, 10%, 1.0 mm
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Novex™ Zymogram Plus (Gelatin) Protein Gels, 10%, 1.0 mm

Novex 10% Zymogram Plus (Gelatin) gels are useful for the detection and characterization of proteases that use gelatin as a substrate. The proteases are run under denaturing conditions and visualized as clear bands against a dark background using a simple renaturing, developing, and staining protocol.
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카탈로그 번호웰 수
ZY00100BOX10-well
ZY00102BOX12-well
ZY00105BOX15-well
카탈로그 번호 ZY00100BOX
제품 가격(KRW)
314,000
Online offer
Ends: 31-Mar-2026
358,000
할인액 44,000 (12%)
10 gels (1 box)
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웰 수:
10-well
제품 가격(KRW)
314,000
Online offer
Ends: 31-Mar-2026
358,000
할인액 44,000 (12%)
10 gels (1 box)
카트에 추가하기
Novex 10% Zymogram Plus (Gelatin) gels are useful for the detection and characterization of proteases that use gelatin as a substrate. The proteases are run under denaturing conditions and visualized as clear bands against a dark background using a simple renaturing, developing, and staining protocol. Zymogram gels are commonly used to detect matrix metalloproteinases. Novex 10% Zymogram Plus gels are highly sensitive, detecting as little as 5 x 10-6 units of collagenase.

Formulation
 Novex Zymogram Plus gels are Tris-glycine gels with 0.1% gelatin incorporated into the gel. They are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, highly purified water, and gelatin as the substrate. All have a 4% stacking gel.

Choose the right gel format for your experiments
Novex 10% Zymogram Plus gels come in a fixed concentration of 10% gelatin and three different well formats, including 10-, 12- and 15-well.

Recommended buffers
 Use Novex Tris-Glycine SDS Sample Buffer (LC2676) and Novex Tris-Glycine SDS Running Buffer (LC2675) with these gels. Novex Zymogram Renaturing Buffer (LC2670) and Novex Zymogram Developing Buffer (LC2671) are also recommended.

For Research Use Only. Not for use in diagnostic procedures.
사양
젤 비율0.1
젤 유형Zymogram Gel
분리 모드Molecular Weight
웰 수10-well
수량10 gels (1 box)
샘플 로딩 부피Up to 25 μL
분리 범위30 to 150 kDa (Tris-Glycine Buffer)
배송 조건Wet Ice
온도(미터법) 보관Store at 2°C to 8°C. Do not freeze.
두께(미터법)1.0 mm
폭(미터법)8 cm
용도 (장비)Mini Gel Tank, XCell SureLock™ Mini-Cell
젤 크기Mini
제품라인Novex
Unit Size10 gels (1 box)

자주 묻는 질문(FAQ)

I ran a Zymogram gel and my bands are blurry and not clear to see. Why is this?

Some small proteases work better if the sample is heated for a short time before loading so the protease is denatured better and it doesn't digest the casein on its way through the gel. This may also help to improve the band sharpness.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What stains do you recommend for Zymogram gels?

Zymogram gels can be stained using Colloidal Blue stain or SimplyBlue SafeStain. The fixing step is not required. We recommend staining with SimplyBlue SafeStain after renaturing and developing the gel for enzyme activity. If greater contrast is needed for detecting weakly active enzymes, a 0.5% Coomassie R250 stain may be used.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Do protein standards run differently on a Zymogram gel compared to a regular Tris-Glycine gel?

Zymogram gels are essentially Tris-Glycine gels containing the substrate. Protein standards run based solely on the percentage of acrylamide and hence should run the same in both kinds of gels. It is quite possible though that if the standard is pre-stained, the proteins will appear a different color because of the staining (or pre-staining) of the Zymogram gels.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Should I boil my sample before running it on a Zymogram gel?

No, samples should not be heated or reduced so that multiunit proteases migrate as a single unit that can be renatured after electrophoresis. The sample should be one part sample and one part 2X Tris-Glycine SDS Sample buffer. Let the sample stand at room temperature for 10 minutes and then load the sample onto the gel.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What are the substrates for the matrix metalloproteases (MMPs)?

The substrates for the matrix metalloproteases (MMPs) are:

*MMP-1: tissue collagenase: collagen 1, 2, 3, 4, 6, & 10

*MMP-2: gelatinase: gelatins, collagens 4, 5, & 7

*MMP-3: stromleysin-2: casein, fibronectin, laminin, elastin

*MMP-7: matrilysin: casein

*MMP-8: neutrophil collagenase: collagen

*MMP-9: type 4 collagenase: gelatin

*MMP-10: stromelysin-2: casein

*MMP-12: metalloelastase: elastin

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

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