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Citations & References (6)
Invitrogen™
RNaseOUT™ Recombinant Ribonuclease Inhibitor
Protects mRNA and improves total cDNA yields.
| Catalog Number | Quantity |
|---|---|
| 10777019 | 5000 Units |
Catalog number 10777019
Price (MXN)
5,856.11
Each
Quantity:
5000 Units
Price (MXN)
5,856.11
Each
RNaseOUT Recombinant Ribonuclease Inhibitor is a potent noncompetitive inhibitor of pancreatic-type ribonucleases such as RNase A and is used to avoid RNA degradation in a variety of applications. It is an acidic protein with a molecular weight of ∼52 kDa that inhibits RNase A, RNase B, and RNase C.
The robust molecular structure of the RNaseOUT™ inhibitor together with a very high binding affinity for pancreatic-type RNases ensures superior protection of RNA from degradation making RNaseOUT™ highly effective and reliable in various molecular biology applications.
Features
- Stable under prolonged reaction conditions—effective RNA protection from RNases at:
- 4°C for at least 96 hours
- 25°C for at least 96 hours
- 37°C for 24 hours
- 50°C for up to 6 hours
- Oxidation resistant—highly stable and requires low concentrations of DTT to retain RNase inhibiting activity
- Wide activity temperature range—maintains full activity from 4°C to 55°C
- Superior quality—stringent quality control and industry leading manufacturing process
Applications
- In vitro transcription and translation
- CRISPR guide RNA production
- RNA purification
- cDNA synthesis
- RT-PCR
- RT-LAMP
- RNA-seq
- RNA modification and labeling
Source
Purified by affinity chromatography from E. coli expressing a cloned porcine gene.
Performance and quality testing
- SDS-PAGE purity
- Endo-deoxyribonuclease assay
- Protein concentration
- Specific activity
- Performance evaluated by RT-PCR
Unit definition
One unit inhibits 5 ng of RNase A by 50% using cytidine 2', 3' cyclic monophosphate (cCMP) as the substrate.
Unit reaction conditions
100 mM Tris-acetate (pH 6.5), 1 mM EDTA, 0.2 mM cCMP, 2 μg RNase A in 1 mL from 0 to 10 min at 25°C
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration40 U/μL
Product LineRNaseOUT
Quantity5000 Units
Unit SizeEach
Contents & Storage
Store at -20°C. Avoid exposing product to frequent changes in temperature. RNaseOUT™ Ribonuclease Inhibitor requires 1 mM DTT to maintain activity.
The product is guaranteed for 6 months from date of purchase unless otherwise stated in the product documentation.
The product is guaranteed for 6 months from date of purchase unless otherwise stated in the product documentation.
Frequently asked questions (FAQs)
Which components of the SuperScript III First Strand Synthesis System for RT-PCR are available for purchase separately?
What does RNaseOUT Recombinant RNase Inhibitor do?
What is the concentration of RNaseOUT Recombinant Ribonuclease Inhibitor in Units/microL?
What is the temperature limitation of RNaseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019)?
I am having trouble performing PCR amplification after DNA isolation with RNAlater-treated samples. Does RNAlater have an impact on downstream PCR applications?
Citations & References (6)
Citations & References
Abstract
The catalytic domain of RNase E shows inherent 3' to 5' directionality in cleavage site selection.
Journal:Proc Natl Acad Sci U S A
PubMed ID:12417756
'RNase E, a multifunctional endoribonuclease of Escherichia coli, attacks substrates at highly specific sites. By using synthetic oligoribonucleotides containing repeats of identical target sequences protected from cleavage by 2''-O-methylated nucleotide substitutions at specific positions, we investigated how RNase E identifies its cleavage sites. We found that the RNase E catalytic
Cloning and functional characterization of HDAC11, a novel member of the human histone deacetylase family.
Journal:J Biol Chem
PubMed ID:11948178
'We have cloned and characterized a human cDNA that belongs to the histone deacetylase family, which we designate as HDAC11. The predicted HDAC11 amino acid sequence reveals an open reading frame of 347 residues with a corresponding molecular mass of 39 kDa. Sequence analyses of the putative HDAC11 protein indicate
Development of reverse transcription (RT)-PCR and real-time RT-PCR assays for rapid detection and quantification of viable yeasts and molds contaminating yogurts and pasteurized food products.
Journal:Appl Environ Microbiol
PubMed ID:12839789
Reverse transcriptase PCR (RT-PCR) and real-time RT-PCR assays have been used to detect and quantify actin mRNA from yeasts and molds. Universal primers were designed based on the available fungal actin sequences, and by RT-PCR they amplified a specific 353-bp fragment from fungal species involved in food spoilage. From experiments
Regulation of H-ras splice variant expression by cross talk between the p53 and nonsense-mediated mRNA decay pathways.
Journal:Mol Cell Biol
PubMed ID:17709397
When cells are exposed to a genotoxic stress, a DNA surveillance pathway that involves p53 is activated, allowing DNA repair. Eukaryotic cells have also evolved a mechanism called mRNA surveillance that controls the quality of mRNAs. Indeed, mutant mRNAs carrying premature translation termination codons (PTCs) are selectively degraded by the
Biochemistry of mitochondrial nitric-oxide synthase.
Journal:J Biol Chem
PubMed ID:12154090
We reported that the generation of nitric oxide by mitochondria is catalyzed by a constitutive, mitochondrial nitric-oxide synthase (mtNOS). Given that this production may establish the basis for a novel regulatory pathway of energy metabolism, oxygen consumption, and oxygen free radical production, it becomes imperative to identify unequivocally and characterize