Sodium pyruvate is commonly added to cell culture media as a carbon source in addition to glucose. Since cells makeRead more
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Catalog Number
Quantity
11360070
100 mL
Catalog number 11360070
Price (MXN)
374.73
Each
Add to cart
Quantity:
100 mL
Price (MXN)
374.73
Each
Add to cart
Sodium pyruvate is commonly added to cell culture media as a carbon source in addition to glucose. Since cells make sodium pyruvate as an intermediate metabolite in the glycolysis pathway, it is not a required supplement for all cell cultures. However, if cells have been grown in medium that is supplemented with sodium pyruvate, we recommend continuing use of the supplement as cell growth may lag without it. Gibco Sodium Pyruvate (100 mM) is formulated using 11 g per liter of water. The final concentration of sodium pyruvate used in most cell culture media is 1 mM.
cGMP Manufacturing and Quality System For supply chain continuity, we manufacture Gibco Sodium Pyruvate (100 mM) at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements and are certified to the ISO 13485 standard.
For In Vitro Diagnostic Use.
Specifications
Cell TypeMammalian Cells
Concentration100 mM
Quantity100 mL
Shelf Life12 Months
Shipping ConditionRoom Temperature
FormLiquid
Product TypeSodium Pyruvate
SterilitySterile-filtered
Unit SizeEach
Contents & Storage
Storage conditions: 2°C to 8°C. Protect from light. Shipping conditions: Room temperature Shelf life: 12 months from date of manufacture
Frequently asked questions (FAQs)
For how long is Sodium Pyruvate (100 mM) stable at 2-8 degrees C after opening the bottle?
When stored properly at 2-8 degrees C, the product is stable for 12 months from date of shipment
Sodium pyruvate (Cat. No. 11360070) is supplied as a liquid supplement for cell culture media at 100 mM (100X) and a final concentration of 11,004 mg/L.
Sodium pyruvate serves as an additional energy source for cells in culture. It is often added to low-glucose formulations (1.0 g/L glucose) and is sometimes added to higher-glucose formulations as well. Cells can become hooked on sodium pyruvate however, and if it is withdrawn suddenly from the media, they may experience a short lag in growth.
A comparison between different human hepatocyte models reveals profound differences in net glucose production, lipid composition and metabolism in vitro.
Authors:Bonanini F,Singh M,Yang H,Kurek D,Harms AC,Mardinoglu A,Hankemeier T
Journal:Experimental cell research
PubMed ID:38499143
Effects of an indole derivative on cell proliferation, transfection, and alternative splicing in production of lentiviral vectors by transient co-transfection.
Authors:Mier NC,Roper DK
Journal:PloS one
PubMed ID:38833479
Lentiviral vectors derived from human immunodeficiency virus type I are widely used to deliver functional gene copies to mammalian cells for research and gene therapies. Post-transcriptional splicing of lentiviral vector transgene in transduced host and transfected producer cells presents barriers to widespread application of lentiviral vector-based therapies. The present study ... More
Metabolism of 4 beta -hydroxycholesterol in humans.
'One of the major oxysterols in the human circulation is 4 beta-hydroxycholesterol formed from cholesterol by the drug-metabolizing enzyme cytochrome P450 3A4. Deuterium-labeled 4 beta-hydroxycholesterol was injected into two healthy volunteers, and the apparent half-life was found to be 64 and 60 h, respectively. We have determined earlier the half-lives ... More
Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O.
Authors:Walev I, Bhakdi SC, Hofmann F, Djonder N, Valeva A, Aktories K, Bhakdi S,
Journal:Proc Natl Acad Sci U S A
PubMed ID:11248053
'The pore-forming toxin streptolysin O (SLO) can be used to reversibly permeabilize adherent and nonadherent cells, allowing delivery of molecules with up to 100 kDa mass to the cytosol. Using FITC-labeled albumin, 10(5)-10(6) molecules were estimated to be entrapped per cell. Repair of toxin lesions depended on Ca(2+)-calmodulin and on ... More
Use of human tissue explants to study human infectious agents.
Authors:Grivel JC, Margolis L,
Journal:Nat Protoc
PubMed ID:19197269
The study of human cell-cell and cell-pathogen interactions that occur in the context of complex tissue cytoarchitecture is critical for deciphering the mechanisms of many normal and pathogenic processes. This protocol describes methods for culturing and infecting explants of human tissues to study the pathogenesis of human infectious agents and ... More