Gateway™ pET-DEST42 Vector

Citations & References (3)

Invitrogen™

Gateway™ pET-DEST42 Vector

The Champion™ pET-DEST42 Gateway™ destination vector is designed for rapid cloning with a Gateway™ entry clone using lambda phage site-specificRead more

Catalog Number	Quantity
12276010	6 μg

Catalog number 12276010

Price (MXN)

Quantity:

The Champion™ pET-DEST42 Gateway™ destination vector is designed for rapid cloning with a Gateway™ entry clone using lambda phage site-specific recombination and subsequent high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter. Expression is induced by the production of T7 RNA polymerase in BL21(DE3) E. coli. The Champion™ pET-DEST42 destination vector offers:

• Bacteriophage T7lac promoter for high-level expression
O operator sequences for lac repressor binding to ensure tighter regulation of transcription
• pBR322 ori for minimal basal expression
• C-terminal V5 and 6xHis tags for efficient detection and purification
R sites for efficient recombination with any attL-flanked Gateway™ entry vector

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Antibiotic Resistance BacterialAmpicillin (AmpR)

Constitutive or Inducible SystemInducible

Inducing AgentIPTG

Product TypeGateway System Destination Expression Vector

Quantity6 μg

Selection Agent (Eukaryotic)None

VectorpET, pDEST

Cloning MethodGateway

Product LineGateway

PromoterT7, lacO

Protein TagHis Tag (6x), V5 Epitope Tag

Unit SizeEach

Contents & Storage

The Champion™ pET-DEST42 Gateway™ destination vector includes 6 μg of vector. Store at -20°C. The vector is guaranteed stable for 6 months when properly stored.

Frequently asked questions (FAQs)

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all Gateway™ pET-DEST42 Vector FAQs

Citations & References (3)

Citations & References

Abstract

Engineering vitamin E content: from Arabidopsis mutant to soy oil.

Authors:Van Eenennaam AL, Lincoln K, Durrett TP, Valentin HE, Shewmaker CK, Thorne GM, Jiang J, Baszis SR, Levering CK, Aasen ED, Hao M, Stein JC, Norris SR, Last RL,

Journal:Plant Cell

PubMed ID:14630966

'We report the identification and biotechnological utility of a plant gene encoding the tocopherol (vitamin E) biosynthetic enzyme 2-methyl-6-phytylbenzoquinol methyltransferase. This gene was identified by map-based cloning of the Arabidopsis mutation vitamin E pathway gene3-1 (vte3-1), which causes increased accumulation of delta-tocopherol and decreased gamma-tocopherol in the seed. Enzyme assays ... More

bZIP transcription factor interactions regulate DIF responses in Dictyostelium.

Authors:Huang E, Blagg SL, Keller T, Katoh M, Shaulsky G, Thompson CR,

Journal:Development

PubMed ID:16410410

The signalling molecule DIF-1 is required for normal cell fate choice and patterning in Dictyostelium. To understand how these developmental processes are regulated will require knowledge of how cells receive and respond to the DIF-1 signal. Previously, we have described a bZIP transcription factor, DimA, which is required for cells ... More

Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis. A novel carboxyl esterase structurally related to the HSL family.

Authors:Canaan S, Maurin D, Chahinian H, Pouilly B, Durousseau C, Frassinetti F, Scappuccini-Calvo L, Cambillau C, Bourne Y,

Journal:Eur J Biochem

PubMed ID:15373841

The Mycobacterium tuberculosis genome contains an unusually high number of proteins involved in the metabolism of lipids belonging to the Lip family, including various nonlipolytic and lipolytic hydrolases. Driven by a structural genomic approach, we have biochemically characterized the Rv1399c gene product, LipH, previously annotated as a putative lipase. Rv1399c ... More