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Thermo Scientific™
Pierce™ Firefly Luciferase Flash Assay Kit
The Thermo Scientific Pierce Firefly Luciferase Flash Assay Kit provides reagents to measure Red firefly luciferase activity in whole cellRead more
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| Catalog Number | Quantity |
|---|---|
| 16174 | 100 Reaction Kit |
| 16175 | 1000 Reaction Kit |
Catalog number 16174
Price (MXN)
3,078.60
Each
Quantity:
100 Reaction Kit
Price (MXN)
3,078.60
Each
The Thermo Scientific Pierce Firefly Luciferase Flash Assay Kit provides reagents to measure Red firefly luciferase activity in whole cell producing a very strong flash signal which decays rapidly.
Features of the Firefly Luciferase Flash Assay Kit:
- Highly sensitive detection of firefly luciferase activity
- Use with Pierce Firefly Luciferase Signal Enhancer for best results (maximum signal)
- Working solution stable for up to 2 months at -20°C
- Assay reagents compatible with other firefly luciferases
- Amenable to automation
- Contains a universal cell lysis buffer and optimized flash assay reagent
- Safe non-radioactive assay
Thermo Scientific Pierce Firefly Luciferase Flash Assay Kit contains reagents to measure the activity of firefly luciferase in mammalian whole cell lysates. Containing a convenient lysis buffer, assay buffer and firefly substrate, this kit provides a complete reagent system for the intracellular detection of promoter or pathway activity. Light output resulting from the luciferase reaction is captured using a luminometer and can be correlated with the amount of firefly luciferase protein produced and used to determine the activity of the promoter driving firefly expression. These reagents are optimized to work with Thermo Scientific Firefly Luc Plasmids; however, the Flash Assay Kit may be used to detect the activity of other firefly or ATP-dependent luciferases utilizing D-luciferin as a substrate.
Includes:
Cell lysis buffer, reaction buffer and substrate; purchase Luciferase Signal Enhancer separately.
Requires:
- Firefly luciferase and luminometer or other instrument capable of monitoring luminescence, such as the Varioskan ALF or the Varioskan LUX microplate readers.
- For best results (maximum signal), use with Pierce Firefly Luciferase Signal Enhancer
Applications:
- Promoter studies for analyzing cis- regulatory elements and trans-acting factors
- Drug screening
- siRNA and miRNA screening
- Multiplexed assays to study off-target effects
- Secretory pathway/protein localization reporter assays
- Signal transduction pathway analysis
- RNA splicing studies
Firefly luciferase is a 60kDa protein produced in nature by several species of the Lampyridae family of beetles which includes the genera Photinus and Luciola. Bioluminescent signal from firefly luciferase originates from the oxidation of D-luciferin. Light output captured using a luminometer can be correlated with the amount of firefly luciferase protein produced and used to determine the activity of the promoter driving firefly expression.
More Product Data
For Research Use Only. Not for use in diagnostic procedures.
Specifications
AssayReporter Enzyme, Luciferase Reporter Assay
Compatible CellsMammalian Cells
Detection MethodBioluminescence
For Use With (Equipment)Luminometer (Microplate)
Format384-well plate, 96-well plate
Label TypeEnzyme Labeled
Product LinePierce
Quantity100 Reaction Kit
SubstrateD-Luciferin
Substrate PropertiesChemical Substrate
Substrate TypeLuciferase Substrate
TargetLuciferase, Firefly Luciferase
TechniqueEnhanced Chemiluminescence
TypeAssay Kit
Unit SizeEach
Contents & Storage
• Firefly Flash Assay Buffer, 5 mL, store at 4°C
• D-Luciferin, Lyophilized, 3 mg, store at 4°C
• 2X Cell Lysis Buffer, 6 mL, store at room temperature
Upon receipt store kit at 4°C or store individual components as indicated above.
• D-Luciferin, Lyophilized, 3 mg, store at 4°C
• 2X Cell Lysis Buffer, 6 mL, store at room temperature
Upon receipt store kit at 4°C or store individual components as indicated above.