Taq DNA Polymerase, native, w/W1

Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.

Catalog Number	Quantity
18038026	500 Units

Catalog number 18038026

Price (MXN)

Quantity:

500 Units

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Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The 1% solution of the detergent W-1 that is included may improve the thermostability of the enzyme when added at a final concentration of 0.05% (v/v).

Taq DNA Polymerase consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5'→3' DNA polymerase activity and a 5'→3' exonuclease activity (see figure).

With Taq DNA Polymerase, you get:

Your choice of recombinant or native enzyme
Amplification of PCR products up to 5 kb in size
An enzyme that is licensed and qualified for PCR

Applications

Amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing

Source

Native enzyme is purified from Thermus aquaticus YT1

Unit definition

One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmol of deoxyribonucleotide into DNA in 30 min. at 74°C.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Exonuclease Activity5' - 3'

Fidelity (vs. Taq)1X

Hot StartNo

Overhang3'-A

PolymeraseTaq DNA Polymerase

Quantity500 Units

Reaction FormatSeparate Components

Shipping ConditionWet or Dry Ice

Size (Final Product)5 kb or less

For Use With (Application)Standard PCR

GC-Rich PCR PerformanceLow

Reaction SpeedStandard

Unit SizeEach

Contents & Storage

• Taq DNA Polymerase, 100 μL
• 10X PCR Buffer (without magnesium), 2.5 mL
• 50 mM MgCl₂, 1 mL
• 1% W-1, 1 mL

Store at -20°C in a non-frost-free freezer.

Frequently asked questions (FAQs)

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

There is a green color in my lyophilized oligo. Can I still use it?

If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

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