Search
Citations & References (3)
Invitrogen™
Biotin-14-dCTP
Biotin-14-dCTP is used for DNA biotinylation, an excellent, and sometimes superior alternative to conventional radioisotopic labeling. Advantages of using biotinylatedRead more
| Catalog Number | Quantity |
|---|---|
| 19518018 | 50 nmol |
Catalog number 19518018
Price (MXN)
-
Quantity:
50 nmol
Biotin-14-dCTP is used for DNA biotinylation, an excellent, and sometimes superior alternative to conventional radioisotopic labeling. Advantages of using biotinylated DNA probes include:
• Convenience and safety—probe preparation generates no radioactive waste
• Stability—probes remain active longer than isotopically labeled probes
• Versatility—biotinylated DNA can be used for immobilization and capture procedures via streptavidin binding
About Biotin-14-dCTP
Biotin-14-dCTP is a patented dCTP analog with biotin attached at the N4-position of the pyrimidine base by a 14-atom linker. This nucleotide can be efficiently incorporated into DNA by nick translation in the presence of dATP, dGTP, and dTTP, labeling DNA with terminal deoxynucleotide transferase, or random primer synthesis. The amount of material provided is sufficient for labeling up to 50 μg of DNA.
Performance and quality testing
Purity is evaluated by reverse-phase HPLC.
• Convenience and safety—probe preparation generates no radioactive waste
• Stability—probes remain active longer than isotopically labeled probes
• Versatility—biotinylated DNA can be used for immobilization and capture procedures via streptavidin binding
About Biotin-14-dCTP
Biotin-14-dCTP is a patented dCTP analog with biotin attached at the N4-position of the pyrimidine base by a 14-atom linker. This nucleotide can be efficiently incorporated into DNA by nick translation in the presence of dATP, dGTP, and dTTP, labeling DNA with terminal deoxynucleotide transferase, or random primer synthesis. The amount of material provided is sufficient for labeling up to 50 μg of DNA.
Performance and quality testing
Purity is evaluated by reverse-phase HPLC.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration0.4 mM
FormLiquid
Labeling MethodDirect Labeling
Label or DyeBiotin & Other Hapten
Product TypeLabeled Nucleotide
Quantity50 nmol
Shipping ConditionDry Ice
Unit SizeEach
Contents & Storage
Biotin-14-dCTP is supplied at 0.4 mM in 100 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Store at -20°C.
Citations & References (3)
Citations & References
Abstract
Novel biotinylated nucleotide--analogs for labeling and colorimetric detection of DNA.
Journal:Nucleic Acids Res
PubMed ID:3588302
'A series of dATP and dCTP nucleotide analogs have been synthesized which are modified by attachment of aliphatic linkers containing a functional group to the amino-nitrogen at the hydrogen bonding positions of the bases, that is, at the 6-position of adenine and the 4-position of cytosine. These nucleotides are incorporated
Differential incorporation of biotinylated nucleotides by terminal deoxynucleotidyl transferase.
Journal:Nucleic Acids Res
PubMed ID:1317552
Comparison of detection and signal amplification methods for DNA microarrays.
Journal:Mol Cell Probes
PubMed ID:18675897
One of the factors limiting the use of DNA microarray technology for the detection of pathogenic organisms from clinical and environmental matrices has been inadequate assay sensitivity. To assess the effectiveness of post-hybridization secondary detection steps to enhance the sensitivity of DNA microarray-based pathogen detection, we evaluated a panel of