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Thermo Scientific™
GelCode™ Blue Stain Reagent
GelCode Blue Stain Reagent is a ready-to-use protein stain based on colloidal coomassie dye G-250 that provides nanogram-level detection in polyacrylamide gels and for in-blot detection.
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| Catalog Number | Quantity |
|---|---|
| 24592 | 3.5 L |
| 24590 | 500 mL |
Catalog number 24592
Price (MXN)
-
Quantity:
3.5 L
GelCode Blue Stain Reagent is a ready-to-use protein stain based on colloidal coomassie dye G-250 that provides nanogram-level detection in polyacrylamide gels and for in-blot detection. This unique reagent stains only protein and allows bands to be viewed directly in the gel during the one-hour gel-staining process.
GelCode Blue Stain Reagent is a ready-to-use protein stain based on colloidal coomassie dye G-250 that provides nanogram-level detection in polyacrylamide gels and for in-blot detection.
Features include:
- No methanol-acetic acid destaining required
- Fast, one-step, one-hour staining, ready-to-use stain reagent; band development visible directly in the staining tray; no background from overnight staining; flexible fixing and washing protocol; no gel shrinkage; stained gel can be dried
- Water wash increases staining sensitivity; optional step provides crystal-clear gel background and makes weakly stained bands more visible
GelCode Blue Stain Reagent stains only protein and allows bands to be viewed directly in the gel during the one-hour gel-staining process. Because the destaining step does not fix the protein, GelCode Blue Stain is compatible with mass spectrometry analysis and N-terminal sequence analysis.
For Research Use Only. Not for use in diagnostic procedures.
GelCode Blue Stain Reagent contains additives that help to slow down the formation of dye-dye and dye-protein aggregates, which form in all coomassie dye-based protein staining reagents. Nevertheless, if left undisturbed, the reagent will form visible dye-dye aggregates that settle in the bottom of the bottle. Fortunately, gently inverting or tipping-and-swirling the bottle several times completely disperses these aggregates. Therefore, it is good practice to mix the stain reagent before pouring or dispensing to ensure that a homogeneous sample of the reagent is used.
Product References:
- Mateer, S.C.,et al.(2002).J. Biol. Chem.227,12324-12333.
- Aulak, K.S.,et al.(2001).Proc. Natl. Acad. Sci. USA98,12056-12061.
- Lim, J.,et al.(2002).J. Biol. Chem.277,20774-20782.
- Hilton, J.M.,et al.(2001).J. Biol. Chem.276,16341-16347.
- Tani, M.,et al.(2002).J. Biol. Chem.275,3462-3468.
Specifications
Detection LocationIn-Blot Detection, In-Gel Detection
Detection MethodColorimetric
Quantity3.5 L
Target MoleculeProtein
ColorBlue
Label or DyeCoomassie
Product LineGelCode
Product TypeProtein Gel Stain
Unit SizeEach
Contents & Storage
Store at 4°C.