SuperSignal™ West Pico Mouse IgG Detection Kit
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Thermo Scientific™

SuperSignal™ West Pico Mouse IgG Detection Kit

Thermo Scientific SuperSignal West Pico Complete Mouse IgG Detection Kit is a a kit containing goat anti-mouse secondary antibody HRPRead more
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Catalog NumberQuantity
34082500 mL
Catalog number 34082
Price (MXN)
-
Quantity:
500 mL
Request bulk or custom format
Thermo Scientific SuperSignal West Pico Complete Mouse IgG Detection Kit is a a kit containing goat anti-mouse secondary antibody HRP conjugate for the detection of mouse primary antibody. The kit is ideal for busy researchers or new beginners to Western blot analysis.

Features of SuperSignal West Pico Complete Mouse IgG Detection Kit:

Optimized—secondary antibodies are validated for use with the SuperSignal West Pico Chemiluminescent Substrate, eliminating the need to test multiple dilutions
Large packages—kits provide sufficient reagents to complete up to 50 experiments with standard mini gel blots (4000 square centimeters of membrane)
Standard or complete—choose a kit with substrate and antibody only, or a complete kit that also includes TBS wash buffer and high-performance SuperBlock Blocking Buffer

All the components of these Western blotting kits are optimized for compatibility the SuperSignal West Pico Chemiluminescent Substrate. The kits are offered in a standard format (substrate and optimized HRP-conjugates) and a complete format that also includes the Thermo Scientific SuperBlock Buffer and BupH Tris Buffered Saline.

Related Products
SuperSignal™ West Pico Complete Mouse IgG Detection Kit
SuperSignal™ West Pico Rabbit IgG Detection Kit
SuperSignal™ West Pico Complete Rabbit IgG Detection Kit
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ConjugateHRP
DescriptionSuperSignal West Pico Mouse IgG Detection Kit
ReactivityMouse
Sufficient For50 mini blots (4000 sq. cm of membrane)
Detection MethodChemiluminescence
For Use With (Application)Western Blot
Membrane CompatibilityNitrocellulose, PVDF
Product LineSuperSignal
Product TypeMouse IgG Detection Kit
Quantity500 mL
SubstrateHorseradish Peroxidase
Unit SizeEach
Contents & Storage
Sufficient For: ∼62 mini blots probed with mouse primary antibodies
• SuperSignal West Pico Luminol/Enhancer Solution, 250 mL
• SuperSignal West Pico Stable Peroxide Solution, 250 mL
• Peroxidase Conjugated Goat anti-Mouse IgG (H + L), 2 mL

Frequently asked questions (FAQs)

How can I increase the sensitivity of my Western blot?

Consider transferring to a different membrane or using a different detection method. We have observed increased sensitivity when using PVDF membranes in place of nitrocellulose. On PVDF membranes, using as little as 1 µg of total rat brain protein, PKC can be detected with alkaline phosphatase-mediated chromogenic detection in some cases using affinity-purified antibodies at a concentration of 0.5 µg/mL. Detection sensitivity can also be increased by using chemiluminescent detection, especially when using a SuperSignal West enhanced chemiluminescence subtrate (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/western-blot-detection-reagents/detection-technologies-western-blotting/chemiluminescent-western-blot-detection/supersignal-chemiluminescent-substrates.html) such as SuperSignal West Pico PLUS, SuperSignal West Dura, or SuperSignal West Femto. The secondary antibody should be used as recommend by the manufacturer and optimized as needed.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.