Sequence Detection Systems Spectral Calibration Kit, 384-well

This Applied Biosystems® easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™, JOE™, NED™,ROX™, SYBR™ Green, TAMRA™,Read more

Catalog Number	Quantity
4323977	2 x 384-well Plates

Catalog number 4323977

Price (MXN)

Quantity:

2 x 384-well Plates

This Applied Biosystems® easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™, JOE™, NED™,ROX™, SYBR™ Green, TAMRA™, TET™, and VIC® dyes on the 7900HT Sequence Detection System. The Sequence Detection Systems Spectral 384-Well Calibration kit includes two optical 384-well reaction plates: one preloaded and sealed background plate, and one preloaded and sealed spectral calibration plate with eight separate dye standards.
• 384-well optical reaction plates preloaded with dyes (FAM™, JOE™, NED™, ROX™, SYBR™ Green, TAMRA™, TET™, and VIC®) are provided for quick and easy calibration of the ABI PRISM® 7900HT Sequence Detection System
• Easy-to-use and provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture
• Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions Spend Your Time on Research—Not Instrument Calibration This kit's easy-to-use, preloaded, 96-well optical reaction plates enable users to calibrate the 384-well block of the 7900HT Sequence Detection System quickly and easily. Insert the reaction plates during system installation, and the system automatically collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Calibrated DyeFAM, JOE, NED, ROX, TAMRA™, TMR (Tetramethylrhodamine), VIC, SYBR™ Green

Calibration TypeSpectral Calibration

Description384-well Plate

Detection MethodFluorescence

For Use With (Application)Real Time PCR (qPCR)

For Use With (Equipment)AB 7900 HT 384-well System

Quantity2 x 384-well Plates

Shipping ConditionDry Ice

Product TypeSpectral Calibration Kit

Unit SizeEach

Contents & Storage

Includes one Background and one Spectral Calibration plate with eight dye standards plates (FAM™, JOE™, NED™, ROX™, SYBR™ Green, TAMRA™, TET™, and VIC™).

Store at -15 to 30°C. Protect from light.

Expiration date: 12 months from the date of delivery

Frequently asked questions (FAQs)

How can I convert a file from AQ to RQ, or from RQ to AQ file with the 7900HT Fast Real-Time PCR System?

SDS v2.4 and above allow you to convert your AQ files to RQ and vice versa.
To do so, simply go to: Tools then AQ RQ Converter
-Choose from the dropdown which direction you want to go (AQ to RQ, or RQ to AQ).
-Next click on ‘Add' and browse to the plate file location in the new window. and click ‘Open'.
-Click on ‘Convert'. A new file will be created in the same location as the original, but with _AQ or _RQ at the end of the filename.

If you do not have v2.4, you can upgrade your software here (http://www.thermofisher.com/us/en/home/technical-resources/software-downloads/applied-biosystems-7900ht-fast-real-timespcr-system.html).

One benefit of this conversion feature is that it allows you to convert 7900 files to the correct type to do analysis for relative quantification study, for example, if a plate is originally run as AQ instead of RQ, or if you would like to generate a standard curve quantification in the AQ if a plate was originally run as RQ.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Why are there no RQ values for all the samples in my study in the 7900HT Fast Real-Time PCR System software?

The 7900HT Fast Real-Time PCR System software requires that the endogenous control be run on every plate within a study. If not, you will only see RQ values for the plate that contains the endogenous control on it. The other plates will have Ct values for all the wells, but the software will not calculate RQ values. If you designed your experiments in this type of layout, you will want to do your analysis in the free DataAssist Software (https://www.thermofisher.com/us/en/home/technical-resources/software-downloads/dataassist-software.html?icid=fr-realtimesoftware-5) instead, or use the Thermo Fisher Cloud (https://www.thermofisher.com/us/en/home/cloud.html). This program does not have the endogenous control requirement for every plate. Simply export the study results file from the 7900HT software as a *.txt or *.csv file, and use that to create a new study in the DataAssist software. You should now be able to see RQ values for all samples, even those without an endogenous control on the same plate.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Why do I not see any Ct or RQ values from my ddCT experiment in the 7900HT Fast Real-Time PCR System software?

In the 7900HT Fast Real-Time PCR System software, v2.3 or later, CT or RQ values are only provided in the RQ Manager software. RQ Manager is installed on the computer along with SDS v2.x.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What can I use for data analysis on the 7900HT Fast Real-Time PCR System?

For genotyping data, we recommend TaqMan Genotyper Software (https://www.thermofisher.com/us/en/home/technical-resources/software-downloads/taqman-genotyper-software.html?icid=fr-software-1).
For relative quantitation, we recommend ExpressionSuite Software (https://www.thermofisher.com/us/en/home/technical-resources/software-downloads/expressionsuite-software.html). You may also use the Thermo Fisher Cloud for either application. https://www.thermofisher.com/us/en/home/cloud.html

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What dyes can I use on the 7900HT Fast Real-Time PCR System?

The Applied Biosystems 7900HT Fast Real-Time PCR Systems use the following dye sets for calibration: FAM, SYBR, VIC, ROX, NED, TAMRA, JOE, TET dyes. Custom dyes can also be used that read between 500 nm and 660 nm, although you will have to calibrate the system first for any new dye.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

View all Sequence Detection Systems Spectral Calibration Kit, 384-well FAQs