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BigDye™ M13 HiRes Primers
Applied Biosystems™

BigDye™ M13 HiRes Primers

The HI-Res Sequencing Primers are optimized primers designed for use with the BigDye™ Terminator BDT Plus Cycle Sequencing Kit to improve sequencing resolution and data quality, particularly at the 5' (five-prime) end of reads.
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Catalog NumberQuantity
4336938100 Reactions
43369391000 Reactions
Catalog number 4336938
Price (MXN)
-
Quantity:
100 Reactions

The HI-Res Sequencing Primers are optimized primers designed for use with the BigDye™ Terminator BDT Plus Cycle Sequencing Kit to improve sequencing resolution and data quality, particularly at the 5' (five-prime) end of reads. Unlike traditional approaches that require dedicated workflows and alternative polymers, HI-Res primers enable enhanced 5' end resolution within standard sequencing workflows using POP-7™ polymer.

Key Features and Benefits

  • Enhance 5' end resolution within standard sequencing workflows using POP-7™ polymer
  • Eliminate the need for dedicated workflows or alternative polymers such as POP-6™
  • Maintain existing workflows with compatibility across BigDye™ Terminator protocols and CE instruments

Product Details

HI-Res Sequencing Primers are specifically engineered to improve sequencing performance at the 5' end of reads, a region that has traditionally been difficult to resolve with standard primer designs. By optimizing primer characteristics and sequencing signal behavior, these primers enable clearer peak resolution and more reliable base calling in early read regions.

Historically, achieving improved 5' end resolution required modified workflows using alternative polymers such as POP-6™, often introducing additional complexity, time, and verification requirements. HI-Res primers enhance 5' end resolution within standard sequencing workflows using POP-7™ polymer.

These primers are designed to work in conjunction with the BigDye™ Terminator BDT Plus Cycle Sequencing Kit, where the combination of optimized primer design and advanced enzyme chemistry enables improved sequencing performance across a wide range of sample types. This integrated system supports consistent results, even when working with challenging templates such as GC-rich regions or structurally complex sequences.

HI-Res primers can be incorporated into current Sanger sequencing workflows. Their compatibility with standard capillary electrophoresis systems and cleanup methods enables easy adoption while enabling improvements in 5' end resolution.

By enabling improved 5' end resolution within existing workflows, HI-Res primers help laboratories generate higher-quality data, simplify operations, and increase overall sequencing efficiency.

For Research Use Only.
Specifications
For Use With (Application)Sanger Sequencing, AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations - 50:50), GC-Rich Sequencing (>65%), Mixed-Base Detection, Long-Read Sequencing, Resequencing, De Novo Sequencing - High Throughput (cDNA), GT-Rich or Difficult Template Sequencing
For Use With (Equipment)SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers
Product LineBigDye Terminator
Product TypeSequencing Kit
Quantity100 Reactions
TechniqueFluorescent Dye Terminator Sequencing
Template CompatibilityBAC DNA, Lambda DNA, Genomic DNA (Bacterial), Single Stranded DNA, Rolling Circle Amplified Products, Fosmids, PCR Amplicons (50:50 Heterozygotes), Plasmid DNA (≤15 Kb), PCR Amplicons
FormatTube
Isolation TechnologyMagnetic Bead
Sequencing TypeGenome and DNA Sequencing
Unit SizeEach

Frequently asked questions (FAQs)

Can I dilute sequencing reactions when using BigDye Terminator Plus Cycle Sequencing Kit?

Yes, sequencing reactions can be diluted when using the BigDye Terminator Plus Cycle Sequencing Kit by using the 5X Sequencing Buffer. When reducing the Ready Reaction Mix volume, additional buffer should be added to maintain a final 1X buffer concentration.
The 5X Sequencing Buffer contains buffer components (for example, Mg²⁺ and Tris) at a higher concentration than the Ready Reaction Mix.
Specifically, the Ready Reaction Mix provides buffer at 2.5X, while the Sequencing Buffer is at 5X, meaning the buffer components are twice as concentrated in the 5X Sequencing Buffer compared to the Ready Reaction Mix. To maintain a final 1X buffer concentration in the sequencing reaction when reducing the volume of Ready Reaction Mix, the volume of 5X Sequencing Buffer added should compensate for the missing buffer. This can be calculated as:

Volume of 5X Sequencing Buffer = ½ × (Nominal Ready Reaction Mix volume – Actual Ready Reaction Mix volume)

Example: For a 10 µL sequencing reaction:
Nominal Ready Reaction Mix volume (full reaction): 4 µL
Actual Ready Reaction Mix volume used: 1 µL
Volume of 5X Buffer = ½ × (4 µL – 1 µL)
Volume of 5X Buffer = ½ × 3 µL = 1.5 µL
In this example, adding 1.5 µL of 5X Sequencing Buffer compensates for the reduced Ready Reaction Mix volume and maintains the correct final buffer concentration for optimal sequencing performance.
This helps to ensure that the overall buffer composition remains consistent despite dilution of the sequencing chemistry.

Can BigDye Terminator Plus Cycle Sequencing Kit be used with dGTP chemistry?

Yes, BigDye Terminator Plus Cycle Sequencing Kit can be used with dGTP chemistry when sequencing challenging templates. When using dGTP, standard reaction setups can be followed, with the addition of a small proportion of dGTP BigDye master mix to the sequencing reaction. Optimization may be required depending on the template.

Is BigDye Terminator Plus Cycle Sequencing Kit compatible with sequencing additives such as DMSO or betaine?

Yes, BigDye Terminator Plus Cycle Sequencing Kit is compatible with commonly used sequencing additives, including GC enhancers, DMSO, betaine, sulfolane, and sequencing enhancer solutions. These additives may be used when working with particularly challenging templates.

How does BigDye Terminator Plus Cycle Sequencing Kit perform on challenging templates such as GC-rich regions?

BigDye Terminator Plus Cycle Sequencing Kit is designed to support sequencing performance across challenging templates, including GC-rich regions, repetitive sequences, and templates with secondary structure.

How does BigDye Terminator Plus Cycle Sequencing Kit compare to BigDye Terminator v3.1 Cycle Sequencing Kit in read length and performance?

BigDye Terminator Plus Cycle Sequencing Kit is designed to support longer read lengths and more consistent signal across challenging templates compared to BigDye Terminator v3.1 Cycle Sequencing Kit, particularly in GC-rich or structured regions.

View all BigDye™ M13 HiRes Primers FAQs