7500 Fast Real-Time PCR System, laptop, QST
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Applied Biosystems™

7500 Fast Real-Time PCR System, laptop, QST

The 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility. It isRead more
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Catalog NumberQuantity
43654641 system
Catalog number 4365464
Price (MXN)
-
Quantity:
1 system
The 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility. It is a sophisticated platform for users who require extended capabilities and maximum versatility. The 3rd generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.

• Powerful, five-color platform is calibrated for the broadest range of dyes available: FAM/SYBR Green I, VIC/JOE, NED/TAMRA/Cy3, ROX/Texas Red, and Cy5 dyes
• Specialized optical system enables easy and accurate calibration to new dyes without requiring the addition of new filter sets
• Advanced multi-componenting algorithm minimizes spectral crosstalk, superior for multiplexing
• User-friendly software includes plate set-up wizards, multi-plate data viewing capabilities, and advanced analysis tools to make data processing simple and straightforward

7500 Software v2.x
Now the easy-to-use StepOne software is available for both the 7500 and 7500 Fast systems with the 7500 Software v2.x upgrade. The 7500 Software v2.x incorporates your favorite StepOne Software features, such as multiple plate setup wizards, calculators for standard curve dilution and master mix recipes, QC flags, data filters, and email notification when a run is finished. The 7500 Software v2.x also includes an enhanced Gene Expression Study package and has a variety of new melting curve protocol options, including multiple peak detection, step and hold temperature control, and customizable ramp rates.

Gene Expression Study package accommodates large studies better than any other instrument software package
• Import an unlimited number of Comparative CT (relative quantitation) files to one study
• Group samples and view data both by technical replicate group and biological replicate group
• Use any gene(s) as an endogenous control, including averaging multiple controls together
• Enter known efficiency values to be factored into the RQ results

21 CFR Part 11 Module available
The SDS v1.4 21CFRp11 Module is a powerful tool for assisting with 21CFRp11 compliance, while still offering the flexibility of user-customizable configuration settings.

• Individual user log-ins can be added for up to four user groups, each group with designated permission settings
• User-customizable permission settings include fourteen system activities, e-signature authority designation, and additional security settings to give you maximum control over your compliance efforts
• Audit trails can be enabled or disabled depending on your traceability needs
• A selection of e-signatures is available to ease e-signatures into your workflow

Supports Many Applications
Applications include gene expression analysis, pathogen quantitation, SNP genotyping, isothermal and +⁄– assays utilizing internal positive controls. To facilitate many of these applications, we provide preformulated, ready-to-use, quality-tested, TaqMan assays for use with the 7500 system. Now you can reduce your assay optimization efforts.

Upgrade to High-Speed Thermal Cycling
An optional upgrade to a the 7500 Fast System is available. This 7500 Fast System uses our master mix formulations and enables you to shorten your real-time PCR runs to as little as 30 minutes.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Calibrated DyeSYBR™ Green I, ROX™, NED™, TAMRA™, JOE™, Texas Red™, Cy5™, VIC™, FAM™, Cy3™
Data AcquisitionCollects Data for all 5 Filters for all Wells regardless of Plate Setup
Dimensions34 cm (W) x 49 cm (H) x 45 cm (D) (13.39 in. x 19.29 in. x 17.72 in.)
Dynamic Range9 Logs of Linear Dynamic Range
Electrical Requirements950 W
External ComputerMinitower
For Use With (Equipment)7500 Fast System
Format96-well Plate
High-throughput CompatibilityHigh-throughput Compatible, Multiplexing
OpticsTungsten-halogen Lamp, Five-Emission Filters and CCD Camera, Five-Excitation Filters
Passive Reference DyeROX (Separate Tube), ROX (Pre-mixed)
PrecisionCan distinguish between 5000 and 10000 Template Copies with 99.7% Confidence
Quantity1 system
Reaction SpeedFast, Standard
Reaction Volume Range10-30 μL
Sample Ramp RateFast Mode: ± 2.2°C⁄s, Standard mode: ± 1.6°C⁄s
SensitivityDown to 1 Copy of Human RNAse P gene
Software CompatibilityPrimer Express Software, System Software
Temperature Range (Metric)4 to 100°C
Thermal AccuracyWithin 0.5°C (35° to 95°C) of Setpoint/Display Temp. (3 min. after start)
Thermal Cycling SystemPeltier-based System
Thermal UniformityWithin 1.0°C (30 s after Clock Start)
ThroughputManual Loading of four-five 96-well Plates per 8-hr day (Real Time throughput)
Voltage120 V
Weight34 kg (75 lb.)
Unit SizeEach
Contents & Storage
Laptop

Frequently asked questions (FAQs)

What run conditions should I use for the TaqMan® Advanced miRNA assays with the discontinued 7900HT Real-Time PCR System?

We recommend using the same thermal profile as listed for the 7500 and 7500 Fast systems (Table 8) in the TaqMan® Advanced miRNA Assays User Guide: https://assets.thermofisher.com/TFS-Assets%2FLSG%2Fmanuals%2F100027897_TaqManAdv_miRNA_Assays_UG.pdf

Can I import a standard curve for my real-time PCR data into the Design & Analysis Software (v2.x)?

It is possible to import a standard curve into the Design & Analysis Software (v2.x) if you are analyzing results from one of the following Applied Biosystems real-time PCR instruments:
• 7900HT Fast Real-Time PCR System
• 7500/7500 Fast Real-Time PCR System
• StepOne/StepOne Plus Real-Time PCR System
• ViiA 7 Real Time PCR System
• QuantStudio 1 Real-Time PCR System
• QuantStudio 3 & 5 Real-Time PCR System
• QuantStudio 6 & 7 Flex Real-Time PCR System
• QuantStudio 6 & 7 Pro Real-Time PCR Systems
• QuantStudio 12K Flex Real-Time PCR system

Follow the instructions below to import a standard curve for analysis in the Design & Analysis Software (v2.x):
1. Open the data file in the Design & Analysis Software (v2.x).
2. Click on Actions.
3. Click on "Standard Curve Analysis Setting".
4. Select "External Standard Curves" and click on "Import".
5. Browse to the proper .csv file and click "Open".
6. Click "Apply".

What is the difference between a Fast 96-well and standard 96-well block?

There are two main differences between a Fast 96-well and standard 96-well block for the Applied Biosystems real-time PCR systems:
• Reaction well volume: The Fast 96-well blocks have 0.1 mL reaction wells and are compatible with 0.1 mL plates and tubes. The standard 96-well blocks have 0.2 mL reaction wells and are compatible with 0.2 mL plates and tubes.
• Ramp rate: The fast 96-well blocks have a higher maximum ramp rate than standard blocks for the same real-time PCR system. However, both the fast 96-well and standard 96-well blocks are capable of running standard and fast chemistry.

Note: For newer instruments (QuantStudio 3 and 5 Real-Time PCR Systems and later) "0.1 mL" and "0.2 mL" are being used in place of "fast" and "standard" as designations for the two 96-well block formats.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Citations & References (1594)

Citations & References
Abstract
Role of APE1 in differentiated neuroblastoma SH-SY5Y cells in response to oxidative stress: Use of APE1 small molecule inhibitors to delineate APE1 functions
Authors:Jiang, YL; Guo, CL; Fishel, ML; Wang, ZY; Vasko, MR; Kelley, MR
Journal:DNA REPAIR
PubMed ID:
Oxidative DNA damage has been implicated in a number of central nervous system pathologies. The base excision repair (BER) pathway is one of the most important cellular protection mechanisms that respond to oxidative DNA damage. Human apurinic (apyrimidinic) endonuclease/redox effector factor (APE1/Ref1 or APE1) is an essential enzyme in the ... More
Characterization of matrix metalloproteinase-2 and matrix metalloproteinase-9 and their inhibitors in equine granulosa cells in vivo and in vitro
Authors:Sessions, DR; Vick, MM; Fitzgerald, BP
Journal:JOURNAL OF ANIMAL SCIENCE
PubMed ID:
Matrix metalloproteinases (MMP) and tissue inhibitors of MMP (TIMP) regulate tissue remodeling events necessary for ovulation. Thus, changes in MMP and TIMP expression and protein enzyme activity were examined in vivo and in vitro during follicular development and atresia in the horse. Equine granulosa cells and follicular fluid from medium ... More
The time of prenatal immune challenge determines the specificity of inflammation-mediated brain and behavioral pathology.
Authors:Meyer U, Nyffeler M, Engler A, Urwyler A, Schedlowski M, Knuesel I, Yee BK, Feldon J
Journal:J Neurosci
PubMed ID:16672647
Disturbance to early brain development is implicated in several neuropsychiatric disorders including autism, schizophrenia, and mental retardation. Epidemiological studies have indicated that the risk of developing these disorders is enhanced by prenatal maternal infection, presumably as a result of neurodevelopmental defects triggered by cytokine-related inflammatory events. Here, we demonstrate that ... More
Distinct roles for IFN regulatory factor (IRF)-3 and IRF-7 in the activation of antitumor properties of human macrophages.
Authors:Romieu-Mourez R, Solis M, Nardin A, Goubau D, Baron-Bodo V, Lin R, Massie B, Salcedo M, Hiscott J
Journal:Cancer Res
PubMed ID:17079482
When properly activated, macrophages can be tumoricidal, thus making them attractive additions to standard cancer therapies. To this end, tolerance and activity of human autologous IFN-gamma-activated macrophages, produced in large scale for clinical use (MAK cells), have been assessed in pilot trials in cancer patients. In the present study, we ... More
Trps1 plays a pivotal role downstream of Gdf5 signaling in promoting chondrogenesis and apoptosis of ATDC5 cells.
Authors:Itoh S, Kanno S, Gai Z, Suemoto H, Kawakatsu M, Tanishima H, Morimoto Y, Nishioka K, Hatamura I, Yoshida M, Muragaki Y
Journal:Genes Cells
PubMed ID:18363966
Tricho-rhino-phalangeal syndrome (TRPS) is an autosomal dominant skeletal disorder caused by mutations of TRPS1. Based on the similar expression patterns of Trps1 and Gdf5, we hypothesized a possible functional interaction between these two molecules. Using a chondrogenic cell line (ATDC5), we investigated the association of Gdf5-mediated signaling pathways with ... More
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