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Applied Biosystems™
AgPath-ID™ One-Step RT-PCR Reagents with manual
AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverseRead more
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| Catalog Number | No. of Reactions |
|---|---|
| 4387424M | 500 Reactions |
| 4387391M | 1000 Reactions |
| AM1005M | 100 Reactions |
Catalog number 4387424M
Price (MXN)
-
No. of Reactions:
500 Reactions
AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.
• Recommended for RNA pathogen amplification
• Consistent amplification of RNA targets with high specificity and sensitivity
• Optimized to work with your target-specific primers and probes
• Contains ROX for quantitative fluorescent signal normalization
AgPath-ID™ One-Step RT-PCR Reagents are configured for fast and simple reaction setup. The reactions are assembled in a single tube, minimizing sample handling errors and expediting set-up time. Once assembled, results are available in approximately one hour. The 25X RT-PCR Enzyme Mix included in the kit contains highly efficient ArrayScript™ Reverse Transcriptase, a mutant MMLV RT that produces high cDNA yields, and AmpliTaq Gold™ polymerase, the preferred hot-start DNA polymerase for specific target amplification. The 2X RT-PCR Buffer has been optimized for efficient, robust reverse transcription and PCR includes the passive reference dye, ROX™ Dye, for quantitative fluorescent signal normalization.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionWorks on all AB platforms, but is optimized for the 7500.
Fidelity (vs. Taq)25 X
For Use With (Equipment)7500 System
FormatTube
No. of Reactions500 Reactions
Passive Reference DyeROX (Pre-mixed)
PolymeraseAmpliTaq Gold DNA Polymerase
Product LineAgPath-ID, Ambion
Product TypeOne-Step RT-PCR Reagent
Quantity500 Reactions
Reverse TranscriptaseArrayScript™
Sufficient For500 Reactions
Volume7 mL
Concentration25X
Detection MethodPrimer-probe
For Use With (Application)Animal Health, Pathogen Detection
GC-Rich PCR PerformanceHigh
PCR Method1-step RT-qPCR
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
Sufficient for 500 reactions of 25 μL, contains:
• 7 mL 2 x RT-PCR Buffer
• 550 μL 25 x RT-PCR Enzyme Mix
• 25 mL Nuclease-free Water.
• 7 mL 2 x RT-PCR Buffer
• 550 μL 25 x RT-PCR Enzyme Mix
• 25 mL Nuclease-free Water.