Pierce™ HeLa Protein Digest Standard
Pierce™ HeLa Protein Digest Standard
Citations & References (3)
Thermo Scientific™

Pierce™ HeLa Protein Digest Standard

The Thermo Scientific Pierce HeLa Protein Digest Standard is a highly validated mammalian protein digest that may be used asRead more
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Catalog NumberQuantity
883295 x 20 μg
8832820 μg
Catalog number 88329
Price (MXN)
-
Quantity:
5 x 20 μg
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The Thermo Scientific Pierce HeLa Protein Digest Standard is a highly validated mammalian protein digest that may be used as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples.

Features of HeLa Protein Digest Standard:

• Positive control sample—complex mammalian proteome sample protein digest (>15,000 proteins) from the HeLa S3 cell line
• Thorough tryptic digestion—prepared using both LysC and trypsin to ensure less than 10% tryptic missed cleavages
• Validated peptide quality—less than 10% methionine oxidation and less than 10% lysine carbamylation
• Rigorously tested—high-quality, consistent protein digest guaranteed and documented via lot-specific certificates of analysis
• Stable—provided in a stable lyophilized format

The protein digest standard is derived from a well-established adenocarcinoma reference HeLa S3 cell line. HeLa S3 cells express over 15,000 proteins with relevant post-translational modifications, making this cell line an ideal standard for complex proteome mass spectrometry applications. The cell lysate has been digested with both LysC and trypsin to minimize tryptic missed cleavages and improve protein sequence coverage. Moreover, unlike other commercially available protein digests for MS, the Pierce HeLa Protein Digest Standard meets stringent quality testing specifications, including peptide quality, digestion efficiency, and lot-to-lot digest uniformity.

Applications:
• Qualitative LC assessment
• LC/MS standardization
• LC/MS method development
• TMT™ Reagent method development

The Pierce HeLa Protein Digest Standard is a lyophilized tryptic peptide mixture that can be used as a quality control standard for liquid chromatography (LC) separation, MS method development and MS performance benchmarking. The digest is specifically formulated for LC/MS experiments and does not contain salts or detergents. Using the digest standard routinely before analysis of complex samples makes it possible to monitor and normalize LC/MS performance between samples and over time.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Final Product TypePeptides
For Use With (Equipment)Mass Spectrometer
Quantity5 x 20 μg
Reagent TypeStandard
Workflow StepMS System Performance
Detection MethodMass Spectrometry
FormSolid
Product LinePierce
Product TypeProtein Digest Standard
Starting MaterialPeptides
Unit SizeEach
Contents & Storage
0.0

Frequently asked questions (FAQs)

How do I verify the LC-MS system after installation of a new column for proteomic applications?

Several technical replicate injections of a known standard sample, e.g., Pierce BSA Digest (Cat. No. 88341), Pierce 6 Protein Digest (Cat. No. 88342), or Pierce HeLa Protein Digest Standard (Cat. Nos. 88328, 88329) should be used to equilibrate and compare the new column to reference chromatograms.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

How do I assess my LC-MS system performance?

The best way to assess the performance of an entire LC-MS system is using a mass spectrometry calibration solution, peptide retention time standards (Pierce Peptide Retention Time Calibration Mixture (PRTC), Cat. No. 88321 and Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix), Cat. No. A40010), and a known standard sample, e.g., Pierce BSA Digest (Cat. No. 88341), Pierce 6 Protein Digest (Cat. No. 88342), or Pierce HeLa Protein Digest Standard (Cat. Nos. 88328, 88329) specific to your application. We recommend frequent assessment of LC-MS system performance on a routine basis to compare results over time.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Which standards and calibrants do you recommend using for assessing LC-MS system suitability?

Here are our recommendations:

- Pierce Peptide Retention Time Calibration Mixture (Cat. No. 88321) for LC system and gradient optimization
- Pierce BSA Protein Digest, MS grade (Cat. No. 88341), Pierce 6 Protein Digest, equimolar, LC-MS grade (Cat. No. 88342), and Pierce HeLa Protein Digest Standard (Cat. Nos. 88328, 88329) for "bottom up" LC-MS method optimization and LC-MS suitability assessment
- Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix) (Cat. No. A40010) for assessing the gradient, sensitivity, and dynamic range of the LC-MS system for discovery and targeted peptide quantitative workflows
- Pierce TMT11plex Yeast Digest Standard (Cat. Nos. A40938, A40939) for assessing system performance for TMT-based quantitative workflows
- Pierce Intact Protein Standard Mix (Cat. Nos. A33526, A33527) for "top down" LC-MS method optimization and LC-MS suitability assessment

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

I'm struggling with mass spectrometry sample clean-up and feel that I'm losing peptides. Can you help me verify?

We recommend using the Pierce HeLa Protein Digest Standard (Cat. Nos. 88328, 88329) to test your sample clean-up method. Use it directly and also as a control, co-treated with the sample to check for any loss of peptides.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

I have a low number of protein identifications from my protein digest sample. What can I do?

We recommend checking your mass spectrometry system performance using the Pierce HeLa Protein Digest Standard (Cat. Nos. 88328, 88329) to help determine if the problem is from the sample preparation or the LC-MS system.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Citations & References (3)

Citations & References
Abstract
QCloud2: An Improved Cloud-based Quality-Control System for Mass-Spectrometry-based Proteomics Laboratories.
Authors:Olivella R,Chiva C,Serret M,Mancera D,Cozzuto L,Hermoso A,Borràs E,Espadas G,Morales J,Pastor O,Solé A,Ponomarenko J,Sabidó E
Journal:Journal of proteome research
PubMed ID:33724836
QCloud is a cloud-based system to support proteomics laboratories in daily quality assessment using a user-friendly interface, easy setup, and automated data processing. Since its release, QCloud has facilitated automated quality control for proteomics experiments in many laboratories. QCloud provides a quick and effortless evaluation of instrument performance that helps ... More
BoxCar and Library-Free Data-Independent Acquisition Substantially Improve the Depth, Range, and Completeness of Label-Free Quantitative Proteomics.
Authors:Mehta D,Scandola S,Uhrig RG
Journal:Analytical chemistry
PubMed ID:34978796
Data-dependent acquisition (DDA) methods are the current standard for quantitative proteomics in many biological systems. However, DDA preferentially measures highly abundant proteins and generates data that is plagued with missing values, requiring extensive imputation. Here, we demonstrate that library-free BoxCarDIA acquisition, combining MS1-level BoxCar acquisition with MS2-level data-independent acquisition (DIA) ... More
Tailoring to Search Engines: Bottom-Up Proteomics with Collision Energies Optimized for Identification Confidence.
Authors:Révész Á,Milley MG,Nagy K,Szabó D,Kalló G,Csősz É,Vékey K,Drahos L
Journal:Journal of proteome research
PubMed ID:33284634
Bottom-up proteomics relies on identification of peptides from tandem mass spectra, usually via matching against sequence databases. Confidence in a peptide-spectrum match can be characterized by a score value given by the database search engines, and it depends on the information content and the quality of the spectrum. The latter ... More