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Citations & References (7)
Thermo Scientific™
RIPA Lysis and Extraction Buffer
Thermo Scientific RIPA Lysis and Extraction Buffer is a high-quality, ready-to-use and fully disclosed formulation of a popular cell lysisRead more
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| Catalog Number | Quantity |
|---|---|
| 89901 | 250 mL |
| 89900 | 100 mL |
Catalog number 89901
Price (MXN)
-
Quantity:
250 mL
Thermo Scientific RIPA Lysis and Extraction Buffer is a high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent for cultured mammalian cells.
Features of RIPA Buffer:
• Convenient—ready-to-use solution; no need to assemble and prepared components yourself
• Flexible—compatible with many applications, including reporter assays, protein assays, immunoassays and protein purification
• Versatile—enables extraction of cytoplasmic, membrane and nuclear proteins
• Disclosed formulation—contains no proprietary components, providing users with complete control and knowledge of possible compatibility issues
This RIPA buffer effectively lyses and extracts protein from cultured mammalian cells, including plated cells and pelleted suspension cells. The popular reagent enables the extraction of membrane, nuclear and cytoplasmic proteins and is compatible with many applications, including reporter assays, the Thermo Scientific BCA Protein Assay, immunoassays and protein purification. Inhibitors such as Thermo Scientific Halt Protease Inhibitor Cocktail (Part No. 78430) and Halt Phosphatase Inhibitor Cocktail (Part No. 78420) are also compatible with this RIPA buffer formulation and can be added before use to prevent proteolysis and maintain protein phosphorylation.
RIPA buffer derives its name from the original application for which it was developed: the radio-immunoprecipitation assay. While this isotopic assay method is rarely performed in laboratories today, the acronym for this lysis buffer formulation has endured in common use. RIPA cell lysis reagent is highly effective for protein extraction from a variety of cell types because it contains three non-ionic and ionic detergents. One disadvantage of this detergent formulation is its relative incompatibility with certain downstream applications compared to other lysis reagents.
Related Products
Pierce™ IP Lysis Buffer
M-PER™ Mammalian Protein Extraction Reagent
Features of RIPA Buffer:
• Convenient—ready-to-use solution; no need to assemble and prepared components yourself
• Flexible—compatible with many applications, including reporter assays, protein assays, immunoassays and protein purification
• Versatile—enables extraction of cytoplasmic, membrane and nuclear proteins
• Disclosed formulation—contains no proprietary components, providing users with complete control and knowledge of possible compatibility issues
This RIPA buffer effectively lyses and extracts protein from cultured mammalian cells, including plated cells and pelleted suspension cells. The popular reagent enables the extraction of membrane, nuclear and cytoplasmic proteins and is compatible with many applications, including reporter assays, the Thermo Scientific BCA Protein Assay, immunoassays and protein purification. Inhibitors such as Thermo Scientific Halt Protease Inhibitor Cocktail (Part No. 78430) and Halt Phosphatase Inhibitor Cocktail (Part No. 78420) are also compatible with this RIPA buffer formulation and can be added before use to prevent proteolysis and maintain protein phosphorylation.
RIPA buffer derives its name from the original application for which it was developed: the radio-immunoprecipitation assay. While this isotopic assay method is rarely performed in laboratories today, the acronym for this lysis buffer formulation has endured in common use. RIPA cell lysis reagent is highly effective for protein extraction from a variety of cell types because it contains three non-ionic and ionic detergents. One disadvantage of this detergent formulation is its relative incompatibility with certain downstream applications compared to other lysis reagents.
Related Products
Pierce™ IP Lysis Buffer
M-PER™ Mammalian Protein Extraction Reagent
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatLiquid
Quantity250 mL
Volume (Metric)250 mL
Product TypeExtraction Buffer
Unit Size250 mL
Contents & Storage
Upon receipt store at 4°C.
Frequently asked questions (FAQs)
What are the standard lysis buffers used with mammalian cells for detection of protein expression by immunoprecipitation (IP) or Western blot analysis?
Does RIPA Lysis and Extraction Buffer (Cat. No. 89900, 89901) contain protease or phosphatase inhibitors?
Why is it not recommended that I use RIPA buffer for protein A280 measurements with my NanoDrop spectrophotometer?
Why is there low phosphorylation of the proteins when I use the RIPA Lysis and Extraction Buffer?
What if proteolysis occurs when I use RIPA Lysis and Extraction Buffer?
Citations & References (7)
Citations & References
Abstract
Not just fat: investigating the proteome of cetacean blubber tissue.
Journal:Conservation physiology
PubMed ID:29479430
Mammalian adipose tissue is increasingly being recognized as an endocrine organ involved in the regulation of a number of metabolic processes and pathways. It responds to signals from different hormone systems and the central nervous system, and expresses a variety of protein factors with important paracrine and endocrine functions. This
Comparison of methods to isolate proteins from extracellular vesicles for mass spectrometry-based proteomic analyses.
Journal:Analytical biochemistry
PubMed ID:31401005
Extracellular vesicles (EVs) are cell-derived membrane-bound organelles that have generated interest as they reflect the physiological condition of their source. Mass spectrometric (MS) analyses of protein cargo of EVs may lead to the discovery of biomarkers for diseases. However, for a comprehensive MS-based proteomics analysis, an optimal lysis of the
Alternative direct-to-amplification cell lysis techniques for forensically relevant non-sperm cells.
Journal:Journal of forensic sciences
PubMed ID:37779342
While efforts have been made to reduce the pervasive backlog of sexual assault evidence collection kits, the actual laboratory process remains very time-consuming due to the requirement of a differential lysis step before DNA purification, as well as intricate mixture analysis towards the end of the DNA workflow. Recently, an
TGFB1-induced extracellular expression of TGFBIp and inhibition of TGFBIp expression by RNA interference in a human corneal epithelial cell line.
Journal:Invest Ophthalmol Vis Sci
PubMed ID:20881301
'To report the increased production of extracellular transforming growth factor ß-induced protein (TGFBIp) by human corneal epithelial cells (HCECs) after induction by TGFB1 and the inhibition of TGFBIp production in induced and noninduced HCECs by RNA interference (RNAi).'
Repair of full-thickness femoral condyle cartilage defects using allogeneic synovial cell-engineered tissue constructs.
Journal:Osteoarthritis Cartilage
PubMed ID:19128988
Synovium-derived stem cells (SDSCs) have proven to be superior in cartilage regeneration compared with other sources of mesenchymal stem cells. We hypothesized that conventionally passaged SDSCs can be engineered in vitro into cartilage tissue constructs and the engineered premature tissue can be implanted to repair allogeneic full-thickness femoral condyle cartilage