Amplex™ Red Xanthine/Xanthine Oxidase Assay Kit

Citations & References (13)

Invitrogen™

Amplex™ Red Xanthine/Xanthine Oxidase Assay Kit

Suitable for cell viability, proliferation and Function, cellular imaging, and enzyme and protein activity assays

Catalog Number	Quantity
A22182	400 Assays

Catalog number A22182

Price (MXN)

Quantity:

400 Assays

The Amplex Red Xanthine/Xanthine Oxidase Assay Kit provides a sensitive and simple fluorometric method for detecting as little as 200 nM xanthine or hypoxanthine or as little as 0.1 mU/mL of xanthine oxidase activity in a purified system with a 100 μL assay volume.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection MethodFluorescence

Dye TypeAmplex Red

Format96-well plate

Quantity400 Assays

Shipping ConditionRoom Temperature

Emission Wavelength Range585 nm

Excitation Wavelength Range571 nm

For Use With (Application)Enzyme and Metabolic Activity

For Use With (Equipment)Microplate Reader, Spectrophotometer, Fluorometer

Product LineAmplex

Product TypeAmplex Red Assay Kit

Unit SizeEach

Contents & Storage

Store in freezer -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

I'm using an Amplex Red kit, the reagent changes color to pink almost immediately in my own Krebs-Ringer buffer but not in HBSS. Why is this?

The components of Krebs-Ringer buffer (salts) should not cause oxidation of the Amplex reagent (which, in the presence of peroxidase and H₂O₂ oxidizes to resorufin, which is pink in color and fluorescent). Try water alone (the water used to make the Krebs-Ringer buffer). Since Hank's Buffered Saline Solution is typically purchased rather than made in the lab, it likely would not have the same contaminant. Another option is to degas the buffer prior to use to removed dissolved oxygen radicals.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can Amplex Red Assays be performed using cell lysates?

This is not recommended. The presence of endogenous proteases can complicate the assay by degrading the horseradish peroxidase (HRP). Endogenous peroxidases and antioxidants can modify the H₂O₂ required for the reaction, competing with HRP (and catalase) for the substrate.

The Amplex Red Assays are best performed with either purified enzymes or extracted H₂O₂ in a defined buffer system, extracellular solutions or body fluids (media, serum, etc.) that do not exhibit high levels of endogenous protease or oxidase activity and do not contain antioxidants.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (13)

Citations & References

Abstract

The novel inosine analogue, INO-2002, protects against diabetes development in multiple low-dose streptozotocin and non-obese diabetic mouse models of type I diabetes.

Authors:Mabley JG, Pacher P, Murthy KG, Williams W, Southan GJ, Salzman AL, Szabo C,

Journal:J Endocrinol

PubMed ID:18562629

Endogenous purines including inosine have been shown to exert immunomodulatory and anti-inflammatory effects in a variety of disease models. The dosage of inosine required for protection is very high because of the rapid metabolism of inosine in vivo. The aim of this study was to determine whether a metabolic-resistant purine ... More

Xanthine oxidase contributes to mechanical ventilation-induced diaphragmatic oxidative stress and contractile dysfunction.

Authors:Whidden MA, McClung JM, Falk DJ, Hudson MB, Smuder AJ, Nelson WB, Powers SK,

Journal:J Appl Physiol (1985)

PubMed ID:18974366

'Respiratory muscle weakness resulting from both diaphragmatic contractile dysfunction and atrophy has been hypothesized to contribute to the weaning difficulties associated with prolonged mechanical ventilation (MV). While it is clear that oxidative injury contributes to MV-induced diaphragmatic weakness, the source(s) of oxidants in the diaphragm during MV remain unknown. These ... More

Voluntary wheel running restores endothelial function in conduit arteries of old mice: direct evidence for reduced oxidative stress, increased superoxide dismutase activity and down-regulation of NADPH oxidase.

Authors:Durrant JR, Seals DR, Connell ML, Russell MJ, Lawson BR, Folian BJ, Donato AJ, Lesniewski LA,

Journal:J Physiol

PubMed ID:19417091

'Habitual aerobic exercise is associated with enhanced endothelium-dependent dilatation (EDD) in older humans, possibly by increasing nitric oxide bioavailability and reducing oxidative stress. However, the mechanisms involved are incompletely understood. EDD was measured in young (6-8 months) and old (29-32 months) cage control and voluntary wheel running (VR) B6D2F1 mice. ... More

Oxidizing Effects of Exogenous Stressors in Huntington's Disease Knock-in Striatal Cells--Protective Effect of Cystamine and Creatine.

Authors:Ribeiro M, Silva AC, Rodrigues J, Naia L, Rego AC,

Journal:

PubMed ID:24008831

'Huntington''s disease (HD) is a polyglutamine-expansion disease associated to degeneration of striatal and cortical neurons. Previously, we showed that oxidative stress occurs in HD knock-in striatal cells, but little is known regarding cell antioxidant response against exogenous stimuli. Therefore, in the present study we analyzed cellular antioxidant profile following hydrogen ... More

Preserved immune functions and controlled leukocyte oxidative stress in naturally long-lived mice: possible role of nuclear factor kappa B.

Authors:Arranz L, Caamaño JH, Lord JM, De la Fuente M,

Journal:J Gerontol A Biol Sci Med Sci

PubMed ID:20558495

'In order to verify the survival biomarker role of several immune functions, and to determine the oxidation and inflammation mechanisms underlying variability in the aging process, we have investigated a variety of immune functions and oxidative stress parameters as well as activation of the nuclear factor kappa B (NF kappaB) ... More

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