'A nonimmune library of 10(9) human antibody scFv fragments has been cloned and expressed on the surface of yeast, and nanomolar-affinity scFvs routinely obtained by magnetic bead screening and flow-cytometric sorting. The yeast library can be amplified 10(10)-fold without measurable loss of clonal diversity, allowing its effectively indefinite expansion. The ... More
Authors:Michael KL, Taylor LC, Schultz SL, Walt DR
Journal:Anal Chem
PubMed ID:9553489
'Array-based sensors provide an architecture for multianalyte sensing. In this paper, we report a new approach for array fabrication. Sensors are made by immobilizing different reactive chemistries on the surfaces of microspheres. Sensor arrays are prepared by randomly distributing a mixture of microsphere sensors on an optical substrate containing thousands ... More
Interphase cytogenetics in pathology: principles, methods, and applications of fluorescence in situ hybridization (FISH).
Authors:Werner M, Wilkens L, Aubele M, Nolte M, Zitzelsberger H, Komminoth P
Journal:Histochem Cell Biol
PubMed ID:9387931
'Characteristic chromosome aberrations have been identified in various tumors. Fluorescence in situ hybridization (FISH) using specific probes that are generated by vector cloning or in vitro amplification and labeled with fluorescent dyes allow for the detection of these genetic changes in interphase cells. This technique, that is also referred to ... More
Improved immunocytochemical detection of biotinylated probes with Neutralite avidin.
Authors:van Roy N, Mangelschots K, Speleman F
Journal:Trends Genet
PubMed ID:8488564
Adhesive and mammalian transglutaminase substrate properties of Candida albicans Hwp1.
Authors:Staab JF, Bradway SD, Fidel PL, Sundstrom P
Journal:Science
PubMed ID:10066176
The pathogenesis of candidiasis involves invasion of host tissues by filamentous forms of the opportunistic yeast Candida albicans. Morphology-specific gene products may confer proinvasive properties. A hypha-specific surface protein, Hwp1, with similarities to mammalian small proline-rich proteins was shown to serve as a substrate for mammalian transglutaminases. Candida albicans strains ... More