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Fetal Bovine Serum, certified, United States
Gibco™

Fetal Bovine Serum, certified, United States

Important Update: We've recently introduced Premium Plus Fetal Bovine Serum (FBS) specification-based products. These improved products are alternatives to our Certified US origin, New Zealand origin, and Qualified Australia origin SKUs. Product stability, performance, manufacturing, storage, and transport processes are unchanged.
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Catalog NumberQuantityFormat
A316040210 x 50 mLOne Shot
A316040150 mLOne Shot
16000044500 mLBottle
Our improved Premium Plus Fetal Bovine Serum (FBS) features enhanced specifications with unchanged stability, manufacturing, storage, and transport processes.
Catalog number A3160402

Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements

Gibco Sera: Trusted Quality for Consistent Performance

Sera Category: Premium (Performance Plus)

  • Use for a broad range of cell types, especially sensitive cell lines
  • Our most characterized sera with the lowest endotoxin and hemoglobin levels
  • Endotoxin level: ≤5 EU/mL
  • Hemoglobin level: ≤15 mg/dL
  • Origin: United States

Gibco Serum Delivers

  • ISO 13485 certified, processed in FDA registered facilities
  • Triple filtered at 0.1 μm
  • Gibco bottle is easier to use in the hood, reduces the risk of contamination and helps you perform cell culture more consistently
  • Peel-off sticker on label provides handy reference for lot number and expiration date recording in lab notebook
For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.
Specifications
DescriptionFetal Bovine Serum, certified, One Shot™ format, United States
Endotoxin Concentration≤5 EU/mL
Hemoglobin Concentration≤15 mg/dL
ProcessingTriple Filtered at 0.1 μm
Product LineOne Shot
Purity or Quality GradeCertified
Shelf Life5 Years
Shipping ConditionFrozen
SpeciesBovine
AgeFetal
Country of OriginUnited States
FormLiquid
FormatOne Shot
Product TypeFetal Bovine Serum
Quantity10 x 50 mL
Serum TreatmentStandard (Sterile-filtered)
SterilitySterile
Sterilization MethodTriple-filtered, 0.1 μm
Unit SizeEach
Contents & Storage
Storage conditions: ≤-10°C
Shipping conditions: Frozen

Frequently asked questions (FAQs)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How much of the total protein measured in Fetal Bovine Serum (heat inactivated or otherwise) is attributed to albumin?

Our CoAs only capture the total protein content not specifically the amount attributed to albumin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between "qualified" versus "certified" purity grade FBS?

The main difference is in the Quality Control (QC) test specifications. “Qualified” and “Certified” FBS go through the same QC tests but “Certified” FBS has additional QC tests as well as more stringent QC test specifications for endotoxin and hemoglobin levels. The additional QC tests in “Certified” FBS include biochemical and hormonal profiling.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can serum be stored at 2-8 degrees C?

Serum can be kept at 2-8 degrees C for up to 4 weeks.

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

Are there any other packaging configurations available for the new One Shot FBS product?

Yes, we also offer a 10 X 50 mL package, which is equivalent to a 500 mL bottle of FBS.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Fetal Bovine Serum, certified, United States FAQs