Amplex™ Red/UltraRed Stop Reagent

Amplex™ Red/UltraRed Stop Reagent is designed for use with the Amplex™ Red and Amplex™ UltraRed fluorogenic substrates and assay kits. Amplex™ Red/UltraRed Stop Reagent provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point. After addition of the stop reagent, the fluorescence signal remains stable for at least 3 hours.

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Amplex™ UltraRed and Amplex™ Red assay kits are sensitive biomolecular assays based on hydrogen peroxide–generating enzyme systems linked to peroxidase–mediated oxidation of the fluorogenic Amplex™ UltraRed or Amplex™ Red substrates. Typically, detection reactions are performed in microplate wells and are initiated by adding the fluorogenic Amplex™ UltraRed or Amplex™ Red substrate, resulting in a continuous fluorescence increase that proceeds for 30 minutes or more. Ultimately, unknown analyte concentrations are determined by comparing fluorescence intensities measured at a certain time point during the reaction to parallel measurements at the same time point on standard samples of known concentration. Clearly, it is critical to ensure that the timing of the standard and unknown sample measurements is the same.

Amplex™ Red/UltraRed Stop Reagent is designed for use with both the Amplex™ Red and Amplex™ UltraRed fluorogenic substrates and assay kits. It is designed to terminate reactions containing up to 0.1 units/mL of horseradish peroxidase (HRP) and 5 μM hyrogen peroxide. The fluorescent signal then remains stable for at least 3 hours.