Tetracycline Hydrochloride

Tetracycline is a yellow, odorless, crystalline powder. It is commonly used as an agent to activate gene expression from anRead more

Catalog Number	Quantity
A39246	500 mg

Catalog number A39246

Price (MXN)

Quantity:

500 mg

Tetracycline is a yellow, odorless, crystalline powder. It is commonly used as an agent to activate gene expression from an inducible expression plasmid in engineered cells expressing the tetracycline repressor protein. Tetracycline Hydrochloride is the hydrochloride salt of tetracycline.

CAS Number: 64-75-5
Formula: C₂₂H₂₄N₂O₂HCl (molecular weight: 480.91)
Solubility: Soluble in water, DMSO, methanol, and ethanol. For cell culture applications, we recommend reconstituting in water.

Tetracycline Hydrochloride is supplied in a glass vial containing 500 mg with 96% purity.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Recommended StorageStore at room temperature.

Shipping ConditionWet Ice

Physical FormPowder

Quantity500 mg

TypeTetracycline Hydrochloride

Unit SizeEach

Frequently asked questions (FAQs)

Can I use doxycycline instead of tetracycline as an inducer in the Expi293 Inducible Expression System?

Doxycycline may be used as an alternative inducing agent in the Expi293 Inducible System. It is similar to tetracycline in its mechanism of action. Doxycycline has been shown to have a longer half-life than tetracycline (48 hr vs. 24 hr, respectively). If the use of doxycycline is desired with the Expi293 Inducible System, we recommend performing a dose-response curve to determine the optimal concentration for induction.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

How much tetracycline do I need to induce expression in Expi293F Inducible or Expi293F Inducible GnTI- cells?

We recommend starting with a tetracycline concentration of 1 µg/mL to induce the expression of your protein of interest. However, you can modulate the level of expression by treating cells with different amounts of tetracycline. We recommend performing a dose-response curve (0-1 µg/mL; 2-fold dilution) to determine the optimal concentration of tetracycline required to induce your protein of interest.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.