Pierce™ Anti-DYKDDDDK Affinity Resin

Thermo Scientific Pierce Anti-DYKDDDDK Spin Columns provide a fast, convenient method for purification of DYKDDDDK-tagged proteins expressed in in vitro protein expression systems, bacteria, yeast, and mammalian cells. The amino acid sequence DYKDDDDK, commonly known as 'FLAG', is recognized by a high-affinity rat monoclonal antibody (clone L5) that is covalently attached to UltraLink Biosupport, which is a rigid, hydrophilic, highly crosslinked, copolymeric, and porous resin with high coupling capacity.

For protein purification, resin is added to a sample containing DYKDDDDK-tagged proteins with the tag on either the N- or C-terminus. Following capture of DYKDDDDK-tagged proteins, non-specifically bound proteins can be washed away before dissociating bound target proteins with elution buffer.

Features of Pierce Anti-DYKDDDDK Affinity Resin

• Specific—unique base beads and highly specific antibody minimize off-target binding (low non-specific binding)
• High purity—optimized bind-wash-elute protocol enables high purity
• High yield—special antibody conjugation method enables high yield
• Rapid—entire purification protocol typically takes less than 40 mins
• Economical—purification protocol allows multiple reuses
• Flexible—available in multiple formats including bulk loose resin and spin columns

Characteristics of Pierce Anti-DYKDDDDK Affinity Resin

• Composition: anti-DYKDDDDK antibody covalently attached to UltraLink Biosupport, a highly crosslinked rigid support
• Particle size: 50–80 μm
• pH stability: 1–13
• Operating temperature: 2–30°C; do not freeze
• Particle concentration: 50% slurry in phosphate buffered saline, 0.02% sodium azide, pH 7.5
• Binding capacity: ≥3.0 mg DYKDDDDK-tGFP-His protein (∼32 kDa/mL settled resin)

Available formats

• Resin slurries—2 mL, 10 mL, and 100 mL of 50% slurry in PBS with 0.02% sodium azide, pH 7.5
• Spin columns—10 mL spin columns containing 3 mL of settled resin, supplied at 6 mL 50% slurry