EasyPep™ MS Sample Prep Kits
EasyPep™ MS Sample Prep Kits
Citations & References (4)
Thermo Scientific™

EasyPep™ MS Sample Prep Kits

Generate high quality proteomic samples for MS analysis in less than four hours using optimized EasyPep MS Sample Prep kits.
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Catalog NumberDescription
A45733MS Sample Prep Kit
A40006Mini MS Sample Prep Kit
A57864Micro MS Sample Prep Kit
A45734Maxi Sample Prep Kit
A45735Lysis Buffer
A57865Peptide Clean-up Plate
Catalog number A45733
Price (MXN)
-
Description:
MS Sample Prep Kit

EasyPep Mini MS Sample Prep Kits enable efficient and reproducible processing of cultured mammalian cells and tissues for proteomic MS analysis. The kit contains pre-formulated buffers, MS-grade enzyme mix, peptide clean-up columns, and an optimized protocol to generate MS-compatible peptide samples in less than three hours. Kits are optimized to process protein samples from 10–100 μg with a high yield of MS-ready peptides.

• Complete—includes pre-formulated reagents for lysis through digestion, peptide clean-up columns, and an optimized protocol for up to 20 samples
• Optimized—streamlined protocol and reagents minimize the number of steps and time time needed to process samples
• Flexible—reagents and protocol have been verified using cells, plasma, and tissue samples for 10 to 100 μg samples
• Time-saving—sample processing time reduced from more than one day to less than three hours
• Compatible—final preparation is ready for direct MS analysis and other downstream applications, including TMT labeling

The sample preparation of peptides for MS analysis is complex, with numerous steps and varied protocols, resulting in sample variability in both quality and quantity. This kit has been designed to improve reproducibility while saving hands-on and processing time. The number of steps and processing time have been reduced through the addition of Universal Nuclease to reduce viscosity from nucleic acids (replacing sonication), a rapid 'one pot' reduction/alkylation solution for cysteine modification (carbamidomethylation, + 57.02), and a trypsin/Lys-C protease mix for more complete digestion. In addition, the kit includes Peptide Clean-up columns and buffers to prepare detergent-free peptide samples for MS analysis. The optimized reagents and protocol produce high-quality peptides that are compatible with TMT labeling and other downstream applications, or are ready for MS analysis.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionMS Sample Prep Kit
Final Product TypePeptides
For Use With (Equipment)Mass Spectrometer
Label or DyeUnlabeled
Quantity96 Reactions
Shipping ConditionWet Ice
Workflow StepProtein Digestion
Detection MethodMass Spectrometry
FormatKit
Product LineEasyPep
Product TypeSample Preparation Kit
Starting MaterialProtein Samples
Unit SizeEach
Contents & Storage
  • Lysis Solution, 25 mL, store at 4°C
  • Universal Nuclease, 25 kU, store at -20°C
  • Reduction Solution, 7 mL, store at 4°C
  • Alkylation Solution, 7 mL, store at 4°C
  • Enzyme Reconstitution solution, 7 mL, store at 4°C
  • Pierce Trypsin/Lys-C Protease Mix, MS Grade, 5 x 100 μg, store at -20°C
  • Digestion Stop Solution, 7 mL, store at 4°C
  • Peptide Clean-Up Plate, 1 Ea., store at 4°C
  • Wash and Elution Plate, 1 Ea., store at 4°C
  • Sample Prep Plate, 1 Ea., store at 4°C
  • Wash Solution A, 40 mL, store at 4°C
  • Wash Solution B, 3 x 27 mL, store at 4°C
  • Elution Solution, 2 x 20 mL, store at 4°C

Frequently asked questions (FAQs)

Are the EasyPep MS Sample Prep Kits compatible with vacuum pumps/dry-down devices?

As per protocol, peptide samples need to be dried down prior to LC-MS analysis. This step can be performed using vacuum pumps/dry-down devices without any harm caused to the device by the components present in the elution buffer within the EasyPep MS Sample Prep Kits.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

I have a lot of plasma samples to prepare for mass spectrometry analysis. Is there a way to perform abundant protein depletion in a 96-well plate?

The bulk Top14 depletion resin is compatible with a 96-well filter plate such as the Agilent filter microplate (Cat. No. 200957-100). Add 600 µL of resin slurry (50%) to each of the wells in the 96-well filter plate, add 10-20 µL of sample, and incubate with gentle shaking. Depleted samples can be collected in a new 96-well polypropylene plate by centrifugation for 2 mins at 1,000 x g or by using a vacuum manifold.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

After cell lysis, my mass spectrometry sample is very viscous and difficult to pipette. How do I reduce the sample viscosity?

High sample viscosity after lysis is due to release of DNA from the nucleus. Sonication or addition of a nuclease such as the Pierce Universal Nuclease (Cat. No. 88700, 88701, or 88702) can be used to degrade DNA and reduce sample viscosity.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

How do I process yeast samples with EasyPep Mini MS Sample prep Kit?

Add 0.5 mm diameter glass beads to the sample pelleted in Easy Pep Lysis Solution and proceed as recommended in the Easy Pep sample prep protocol. Vortex vigorously for few minutes, spin at 16,000 x g for 5 mins, and extract the supernatant for protein assay and further downstream sample preparation.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

How do I process bacterial samples with EasyPep MS Sample Prep kits?

Add 1-2 µL of 50 mg/mL Lysozyme Solution (Cat. No. 90082) to 200 µL of the EasyPep lysis buffer provided in the kit and proceed with the sample prep. Add 200 µL of the prepared lysis solution to 5 X 10E8 bacterial cells (E. coli cells) (OD600 equal to 1, corresponding to 1 X 10E8 CFUs). Lyse by pipetting the sample repeatedly and centrifuge at 16,000 x g for 5 mins. Alternatively, E. coli cells can be lysed in lysis buffer using bead beating or sonication instead of lysozyme. After lysis, dilute samples to 1 mg/mL using lysis buffer, proceed with the EasyPep protocol for reduction, alkylation, digestion, and clean up as described in the product manual.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

View all EasyPep™ MS Sample Prep Kits FAQs

Citations & References (4)

Citations & References
Abstract
Discovery of Orally Available and Brain Penetrant AEP Inhibitors.
Authors:Krummenacher D,He W,Kuhn B,Schnider C,Beurier A,Brom V,Sivasothy T,Marty C,Tosstorff A,Hewings DS,Mesch S,Pinard E,Brändlin M,Hochstrasser R,Westwood P,Rothe J,Kronenberger A,Morandi F,Gutbier S,Schuler A,Heer D,Gloria LE,Joedicke L,Rudolph MG,Müller L,Grüninger F,Baumann K,Kaniyappan S,Manevski N,Bartels B
Journal:Journal of medicinal chemistry
PubMed ID:38090813
Alzheimer's Disease (AD) is the most widespread form of dementia, with one of the pathological hallmarks being the formation of neurofibrillary tangles (NFTs). These tangles consist of phosphorylated Tau fragments. Asparagine endopeptidase (AEP) is a key Tau cleaving enzyme that generates aggregation-prone Tau fragments. Inhibition of AEP to reduce the ... More
Development of a First-in-Class RIPK1 Degrader to Enhance Antitumor Immunity.
Authors:Wang J,Lu D,Yu X,Qi X,Lin H,Holloman BL,Jin F,Xu L,Ding L,Peng W,Wang M,Chen X
Journal:Research square
PubMed ID:38659866
The scaffolding function of receptor interacting protein kinase 1 (RIPK1) confers intrinsic and extrinsic resistance to immune checkpoint blockades (ICBs) and has emerged as a promising target for improving cancer immunotherapies. To address the challenge posed by a poorly defined binding pocket within the intermediate domain, we harnessed proteolysis targeting ... More
Proteomic Characterization of Transfusable Blood Components: Fresh Frozen Plasma, Cryoprecipitate, and Derived Extracellular Vesicles via Data-Independent Mass Spectrometry.
Authors:Hwang JH,Lai A,Tung JP,Harkin DG,Flower RL,Pecheniuk NM
Journal:Journal of proteome research
PubMed ID:39254217
Extracellular vesicles (EVs) are a heterogeneous collection of particles that play a crucial role in cell-to-cell communication, primarily due to their ability to transport molecules, such as proteins. Thus, profiling EV-associated proteins offers insight into their biological effects. EVs can be isolated from various biological fluids, including donor blood components ... More
Targeted sulfur(VI) fluoride exchange-mediated covalent modification of a tyrosine residue in the catalytic pocket of tyrosyl-DNA phosphodiesterase 1.
Authors:Zhao XZ,Barakat IA,Lountos GT,Wang W,Agama K,Mahmud MRA,Suazo KF,Andresson T,Pommier Y,Burke TR Jr
Journal:Communications chemistry
PubMed ID:39284936
Developing effective inhibitors of the DNA repair enzyme tyrosyl-DNA phosphodiesterase 1 (TDP1) has been challenging because of the enzyme shallow catalytic pocket and non-specific substrate binding interactions. Recently, we discovered a quinolone-binding hot spot in TDP1's active site proximal to the evolutionary conserved Y204 and F259 residues that position DNA. ... More