DiBAC4(3) (Bis-(1,3-Dibutylbarbituric Acid)Trimethine Oxonol)
DiBAC<sub>4</sub>(3) (Bis-(1,3-Dibutylbarbituric Acid)Trimethine Oxonol)
Citations & References (160)
Invitrogen™

DiBAC4(3) (Bis-(1,3-Dibutylbarbituric Acid)Trimethine Oxonol)

The slow-response potential-sensitive probe, DiBAC4(3) can enter depolarized cells where it binds to intracellular proteins or membrane and exhibits enhancedRead more
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Catalog NumberQuantity
B43825 mg
Catalog number B438
Price (MXN)
-
Quantity:
25 mg

The slow-response potential-sensitive probe, DiBAC4(3) can enter depolarized cells where it binds to intracellular proteins or membrane and exhibits enhanced fluorescence and a red spectral shift. Increased depolarization results in additional influx of the anionic dye and an increase in fluorescence. Conversely, hyperpolarization is indicated by a decrease in fluorescence. This bis-oxonal has an excitation maxima of 490 nm and emission maxima of 516 nm. DiBAC dyes are excluded from mitochondria because of their overall negative charge, making them superior to carbocyanines for measuring plasma membrane potentials.

Dissolve in high-quality anhydrous DMSO or ethanol to prepare a stock concentration up to 1 mM.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Dye TypeMembrane Potential Probes
Excitation/Emission493/516 nm
Molecular Weight (g/mol)516.64
Quantity25 mg
Shipping ConditionRoom Temperature
Product TypeDiBAC4(3)
Unit SizeEach
Contents & Storage
Store at room temperature and protect from light.

Frequently asked questions (FAQs)

I am seeing high background outside of my neuronal cells when using membrane potential indicators. What can I do to reduce background?

If you use our FluoVolt Membrane Potential Kit (Cat. No. F10488), the kit provides a background suppressor to reduce this problem. For other indicators, consider the use of BackDrop Background Suppressor (Cat no. R37603, B10511, and B10512).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between fast and slow-response membrane potential probes?

Molecules that change their structure in response to the surrounding electric field can function as fast-response probes for the detection of transient (millisecond) potential changes. Slow-response dyes function by entering depolarized cells and binding to proteins or membranes. Increased depolarization results in additional dye influx and an increase in fluorescence, while hyperpolarization is indicated by a decrease in fluorescence. Fast-response probes are commonly used to image electrical activity from intact heart tissues or measure membrane potential changes in response to pharmacological stimuli. Slow-responding probes are often used to explore mitochondrial function and cell viability.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What type of membrane potential indicators do you offer and how should I choose one for my experiment?

A membrane potential indicator selection guide can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-viability-and-regulation/ion-indicators/membrane-potential-indicators.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (160)

Citations & References
Abstract
A novel membrane potential-sensitive fluorescent dye improves cell-based assays for ion channels.
Authors:Baxter DF, Kirk M, Garcia AF, Raimondi A, Holmqvist MH, Flint KK, Bojanic D, Distefano PS, Curtis R, Xie Y
Journal:J Biomol Screen
PubMed ID:11897058
The study of ion channel-mediated changes in membrane potential using the conventional bisoxonol fluorescent dye DiBAC(4)(3) has several limitations, including a slow onset of response and multistep preparation, that limit both the fidelity of the results and the throughput of membrane potential assays. Here, we report the characterization of the ... More
Characterization of human urinary bladder KATP channels containing SUR2B splice variants expressed in L-cells.
Authors:Scott VE, Davis-Taber RA, Silvia C, Hoogenboom L, Choi W, Kroeger P, Whiteaker KL, Gopalakrishnan M
Journal:Eur J Pharmacol
PubMed ID:14729107
The molecular properties of the sulfonylurea receptor 2 (SUR2) subunits of K(ATP) channels expressed in urinary bladder were assessed by polymerase chain reaction (PCR). This showed that SUR2B exon 17- mRNA (72%) was predominant over the SUR2B exon 17+ splice variant (28%). The pharmacological properties of both of these isoforms ... More
Overexpression of Na(+)/K (+)-ATPase parallels the increase in sodium transport and potassium recycling in an in vitro model of proximal tubule cellular ageing.
Authors:Silva E, Gomes P, Soares-da-Silva P,
Journal:J Membr Biol
PubMed ID:17334838
'Na(+)/K(+)-ATPase plays a key role in the transport of Na(+) throughout the nephron, but ageing appears to be accompanied by changes in the regulation and localization of the pump. In the present study, we examined the effect of in vitro cell ageing on the transport of Na(+) and K(+) ions ... More
TRPC1 associates with BK(Ca) channel to form a signal complex in vascular smooth muscle cells.
Authors:Kwan HY, Shen B, Ma X, Kwok YC, Huang Y, Man YB, Yu S, Yao X,
Journal:Circ Res
PubMed ID:19168436
'TRPC1 (transient receptor potential canonical 1) is a Ca(2+)-permeable cation channel involved in diverse physiological function. TRPC1 may associate with other proteins to form a signaling complex, which is crucial for channel function. In the present study, we investigated the interaction between TRPC1 and large conductance Ca(2+)-sensitive K(+) channel (BK(Ca)). ... More
Lipid factor (bVLF) from bovine vitreous body evokes in EGFR-T17 cells a Ca2+-dependent K+ current associated with inositol 1,4,5-trisphosphate-independent Ca2+ mobilization.
Authors:Camiña JP, Diaz-Rodriguez E, Harks EG, Theuvenet AP, Ypey DL, Casanueva FF
Journal:J Cell Physiol
PubMed ID:12599214
'Bovine vitreous lipid factor (bVLF) is a complex phospholipid isolated from bovine vitreous body with strong Ca(2+)-mobilizing activity. In this study, the effects of bVLF on membrane potential were investigated in EGFR-T17 fibroblasts with the whole-cell patch clamp technique on monolayer cells, as well as with the fluorescent dye bis-oxonol ... More
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