5-(and-6)-Carboxy SNARF™-1, Acetoxymethyl Ester, Acetate

Citations & References (287)

Invitrogen™

5-(and-6)-Carboxy SNARF™-1, Acetoxymethyl Ester, Acetate

Carboxy SNARF™-1, acetoxymethyl ester, acetate is a cell-permeant pH indicator that has a pKa of ∼7.5 after de-esterification; thus, itRead more

Catalog Number	Quantity
C1272	20 x 50 μg

Catalog number C1272

Price (MXN)

Quantity:

20 x 50 μg

Carboxy SNARF™-1, acetoxymethyl ester, acetate is a cell-permeant pH indicator that has a pK_a of ∼7.5 after de-esterification; thus, it is useful for measuring pH changes between pH 7 and pH 8. Carboxy-SNARF™-1 exhibits a significant pH-dependent emission shift from yellow-orange to deep red fluorescence under acidic and basic conditions, respectively. This pH dependence allows the ratio of the fluorescence intensities from the dye at two emission wavelengths - typically 580 nm and 640 nm - to be used for quantitative determinations of pH.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection MethodFluorescence

Dye TypepH Indicator

Quantity20 x 50 μg

Shipping ConditionRoom Temperature

For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Microplate Reader

Product LineSNARF

Product TypepH Indicator

Unit SizeEach

Contents & Storage

Store in freezer -5°C to -30°C.

Frequently asked questions (FAQs)

Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?

Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (287)

Citations & References

Abstract

Simultaneous measurement of intracellular pH and Ca2+ in insulin-secreting cells by spectral imaging microscopy.

Authors:Martínez-Zaguilán R,Gurulé MW,Lynch RM

Journal:The American journal of physiology

PubMed ID:8967445

Molecular cloning and functional expression of a rat Na+/H+ exchanger (NHE5) highly expressed in brain.

Authors:Attaphitaya S, Park K, Melvin JE

Journal:J Biol Chem

PubMed ID:9933642

We report here the cloning, primary structure, heterologous expression, tissue distribution, and localization of a cDNA encoding rat NHE5, a fifth member of the mammalian plasma membrane Na+/H+ exchanger (NHE) gene family. The full-length open reading frame as well as 34 nucleotides of 5'-untranslated and 1443 nucleotides of 3'-untranslated sequences ... More

Measurement of intracellular pH.

Authors:Boyer MJ, Hedley DW

Journal:Methods Cell Biol

PubMed ID:7532259

Simultaneous imaging of intracellular [Ca2+] and pH in single MDCK and glomerular epithelial cells.

Authors:Wiegmann TB, Welling LW, Beatty DM, Howard DE, Vamos S, Morris SJ

Journal:Am J Physiol

PubMed ID:8238309

The interrelationships between changes in intracellular calcium concentration ([Ca2+]i) and intracellular pH in Madin-Darby canine kidney cells and kidney glomerular epithelial cells exposed to various stimuli were analyzed simultaneously using a new design of a fluorescence video microscope. Cells were double labeled with indo 1 and SNARF 1 dyes and ... More

Identification of band 3-like proteins and Cl-/HCO3- exchange in isolated cardiomyocytes.

Authors:Pucéat M, Korichneva I, Cassoly R, Vassort G

Journal:J Biol Chem

PubMed ID:7836397

'The identification of the protein that exerts the function of Cl-/HCO3- exchange is still unresolved in cardiac tissue. We have addressed this issue by using a multiple technical approach. Western blotting analysis with an antibody raised against human erythroid whole band 3 protein, the so-called protein that mediates the Cl-/HCO3- ... More

View all 5-(and-6)-Carboxy SNARF™-1, Acetoxymethyl Ester, Acetate, 20 x 50 μg citations