5(6)-CFDA (5-(and-6)-Carboxyfluorescein Diacetate), mixed isomers

5(6)-CFDA is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which isRead more

Catalog Number	Quantity
C195	100 mg

Catalog number C195

Price (MXN)

Quantity:

100 mg

5(6)-CFDA is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which is require to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product. Upon hydrolysis by intracellular esterases, this AM ester also yields carboxyfluorescein. As compared with fluorescein, carboxyfluorescein contains extra negative charges and is therefore better retained in cells.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Dye TypeOther Label(s) or Dye(s)

Quantity100 mg

Reagent TypeCFDA & Related Compounds

Shipping ConditionRoom Temperature

Product Type5(6)-CFDA

Unit SizeEach

Contents & Storage

Store in freezer -5°C to -30°C.

Citations & References (185)

Citations & References

Abstract

Bacteria in post-glacial freshwater sediments.

Authors:Miskin I, Rhodes G, Lawlor K, Saunders JR, Pickup RW

Journal:Microbiology

PubMed ID:9782490

Prokaryote communities in post-glacial profundal freshwater sediments of Windermere, representing 10-12,000 years of deposition, were examined for culturability, viability and community structure. The potential for active geochemical cycles was inferred from the presence of specific groups of bacteria. Direct count procedures revealed 10(12) cells (g dry wt sediment)-1 in the ... More

5-Carboxyfluorescein diacetate as a probe for measuring the growth of keratinocytes.

Authors:Hanthamrongwit M, Reid WH, Courtney JM, Grant MH

Journal:Hum Exp Toxicol

PubMed ID:8086226

There is a requirement for a convenient and reliable method for evaluating the growth rate of human keratinocytes cultured on collagen-based substrates. Therefore, three methods of determining cell growth were first used to quantify the growth rate of the well-characterised L929 mouse fibroblast cell line on tissue culture plastic and ... More

Fluorescent indicators of ion concentrations.

Authors:Tsien RY

Journal:Methods Cell Biol

PubMed ID:2538708

Rapid assessment of physiological status in Escherichia coli using fluorescent probes.

Authors:Porter J, Edwards C, Pickup RW

Journal:J Appl Bacteriol

PubMed ID:7592133

'Rapid and direct viability assessment of Escherichia coli in filtered, sterile lake water was possible using multiparameter flow cytometry. Fluorescent dyes were used as probes for different cellular functions (membrane potential, membrane integrity and intracellular enzyme activity), which were correlated with the ability of the cells to respond to nutrient ... More

Intercellular adhesion mediated by human muscle neural cell adhesion molecule: effects of alternative exon use.

Authors:Pizzey JA, Rowett LH, Barton CH, Dickson G, Walsh FS

Journal:J Cell Biol

PubMed ID:2532218

'Mouse 3T3 fibroblasts were permanently transfected with cDNAs encoding isoforms of the neural cell adhesion molecule (N-CAM) present in human skeletal muscle and brain. Parental and transfected cells were then used in a range of adhesion assays. In the absence of external shear forces, transfection with cDNAs encoding either transmembrane ... More

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