JC-9 Dye (Mitochondrial Membrane Potential Probe)

Citations & References (5)

Invitrogen™

JC-9 Dye (Mitochondrial Membrane Potential Probe)

The ratiometric mitochondrial membrane potential probe JC-9 exhibits green fluorescence that is independent of membrane potential and an increase inRead more

Catalog Number	Quantity
D22421	5 mg

Catalog number D22421

Price (MXN)

Quantity:

The ratiometric mitochondrial membrane potential probe JC-9 exhibits green fluorescence that is independent of membrane potential and an increase in red fluorescence at hyperpolarized membrane potentials.

Given that JC-1 and JC-9 dyes are mitochondrial membrane potential indicators, they are designed to be used in live cells with active mitochondria. These dyes are not compatible with fixed cell staining.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection MethodFluorescence

Quantity5 mg

Shipping ConditionRoom Temperature

Sub Cellular LocalizationMitochondria

ColorGreen

For Use With (Equipment)Fluorescence Microscope, Flow Cytometer

Product TypeMitochondrial Membrane Potential Probe

Unit SizeEach

Contents & Storage

Store at room temperature and protect from light.

Frequently asked questions (FAQs)

I am seeing high background outside of my neuronal cells when using membrane potential indicators. What can I do to reduce background?

If you use our FluoVolt Membrane Potential Kit (Cat. No. F10488), the kit provides a background suppressor to reduce this problem. For other indicators, consider the use of BackDrop Background Suppressor (Cat no. R37603, B10511, and B10512).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between fast and slow-response membrane potential probes?

Molecules that change their structure in response to the surrounding electric field can function as fast-response probes for the detection of transient (millisecond) potential changes. Slow-response dyes function by entering depolarized cells and binding to proteins or membranes. Increased depolarization results in additional dye influx and an increase in fluorescence, while hyperpolarization is indicated by a decrease in fluorescence. Fast-response probes are commonly used to image electrical activity from intact heart tissues or measure membrane potential changes in response to pharmacological stimuli. Slow-responding probes are often used to explore mitochondrial function and cell viability.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What type of membrane potential indicators do you offer and how should I choose one for my experiment?

A membrane potential indicator selection guide can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-viability-and-regulation/ion-indicators/membrane-potential-indicators.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (5)

Citations & References

Abstract

Synthesis and identification of small molecules that potently induce apoptosis in melanoma cells through G1 cell cycle arrest.

Authors:Dothager RS, Putt KS, Allen BJ, Leslie BJ, Nesterenko V, Hergenrother PJ,

Journal:J Am Chem Soc

PubMed ID:15954774

'Late-stage malignant melanoma is a cancer that is refractory to current chemotherapeutic treatments. The average survival time for patients with such a diagnosis is 6 months. In general, the vast majority of anticancer drugs operate through induction of cell cycle arrest and cell death in either the DNA synthesis (S) ... More

Chlorophenols and chlorocatechols induce apoptosis in human lymphocytes (in vitro).

Authors:Michalowicz J, Sicinska P,

Journal:Toxicol Lett

PubMed ID:19766705

In this work the effect of 2,4,5-trichlorophenol (2,4,5-TCP), pentachlorophenol (PCP), 4,6-dichloroguaiacol (4,6-DCG), tetrachloroguaiacol (TeCG), 4,5-dichlorocatechol (4,5-DCC) and tetrachlorocatechol (TeCC) on the induction of apoptosis in human peripheral blood lymphocytes was examined. The analysis of the changes in mitochondrial transmembrane potential (DeltaPsi(m)) was performed using JC-9 fluorescent probe. It was noted ... More

Markers of apoptosis: methods for elucidating the mechanism of apoptotic cell death from the nervous system.

Authors:Smyth PG, Berman SA,

Journal:Biotechniques

PubMed ID:11911667

Apoptosis is a highly conserved energy-requiring program for non-inflammatory cell death that is important in both normal physiology and disease. Numerous techniques have been used to study apoptosis. In the nervous system, apoptosis is necessary for normal development, but it also occurs in many acute and chronic pathologic conditions. This ... More

DGAT1-Dependent Lipid Droplet Biogenesis Protects Mitochondrial Function during Starvation-Induced Autophagy.

Authors:

Journal:Dev Cell

PubMed ID:28697336

A role for mitochondria in the establishment and maintenance of the maize root quiescent center.

Authors:

Journal:Plant Physiol

PubMed ID:16443698