DiFMU (6,8-Difluoro-7-Hydroxy-4-Methylcoumarin), reference standard

DIFMU can be used as a reference standard for ether, ester and phosphate substrates derived from this blue-fluorescent dye.Read more

Catalog Number	Quantity
D6566	10 mg

Catalog number D6566

Price (MXN)

Quantity:

10 mg

DIFMU can be used as a reference standard for ether, ester and phosphate substrates derived from this blue-fluorescent dye.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label or DyeDiFMU

Product TypeReference Standard

Quantity10 mg

Shipping ConditionRoom Temperature

Label TypeCoumarin Dyes

Unit SizeEach

Contents & Storage

Store at room temperature and protect from light.

Citations & References (4)

Citations & References

Abstract

A fluorogenic substrate for the continuous assaying of aryl sulfatases.

Authors:Ahmed V, Ispahany M, Ruttgaizer S, Guillemette G, Taylor SD,

Journal:Anal Biochem

PubMed ID:15802133

'The most common fluorogenic substrate for assaying aryl sulfatases (ARSs) is 4-methylumbelliferyl sulfate (MUS). However, ARSs operate optimally at pH values that are less than the pK(a) (7.8) of the reaction product of MUS, 4-methylumbelliferone (4-MU). Thus, a major disadvantage of this assay is that it is usually run in ... More

Synthesis of novel fluorinated coumarins: excellent UV-light excitable fluorescent dyes.

Authors:Sun WC, Gee KR, Haugland RP

Journal:Bioorg Med Chem Lett

PubMed ID:9873685

'Two new fluorinated fluorescent dyes, 6,8-difluoro-7-hydroxy-4-methylcoumarin (Marina Blue) and 3-carboxy-6,8-difluoro-7-hydroxycoumarin (Pacific Blue), exhibit excellent photophysical properties among a series of novel fluorinated 7-hydroxycoumarins. Most of these fluorinated coumarins have quantum yields (0.63 to 0.89) equal to or higher than that of the parent compound (0.63), which, in combination with their ... More

Characterization of the histidine-containing phosphotransfer protein B-mediated multistep phosphorelay system in Pseudomonas aeruginosa PAO1.

Authors:Hsu JL, Chen HC, Peng HL, Chang HY,

Journal:J Biol Chem

PubMed ID:18256026

Certain bacterial two-component sensor kinases possess a histidine-containing phosphotransfer (Hpt) domain to carry out a multistep phosphotransferring reaction to a cognate response regulator. Pseudomonas aeruginosa PAO1 contains three genes that encode proteins with an Hpt domain but lack a kinase domain. To identify the sensor kinase coupled to these Hpt ... More

A fluorogenic substrate for detection of organophosphatase activity.

Authors:Soukharev S, Hammond DJ

Journal:Anal Biochem

PubMed ID:15033522

A new fluorogenic substrate for the specific detection of organophosphatase (OPase) activity has been designed and evaluated. Our results indicate that 7-diethylphospho-6,8-difluor-4-methylumbelliferyl (DEPFMU) is hydrolyzed specifically by the OPases, mammalian serum paraoxonase and bacterial organophosphorus hydrolase (OPH). The apparent K(m) of DEPFMU is 29 microM for OPH and 91 and ... More