Total Bile Acid (TBA) Colorimetric Assay Kit
Invitrogen™

Total Bile Acid (TBA) Colorimetric Assay Kit

With this kit, 3α-hydroxy steroid dehydrogenase catalyzes the dehydrogenation of bile acids to produce 3-ketone steroids, transforming S-NAD+ into S-NADH.
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Catalog NumberQuantity
EEA03596 Tests
Catalog number EEA035
Price (MXN)
-
Quantity:
96 Tests

With this kit, 3α-hydroxy steroid dehydrogenase catalyzes the dehydrogenation of bile acids to produce 3-ketone steroids, transforming S-NAD+ into S-NADH. Through the enzyme cycle reaction, S-NADH is continuously generated, which has a maximum absorption peak at 405 nm. Measure the OD value at 405 nm and the change in absorbance is proportional to the concentration of bile acid. This kit is useful for researchers studying liver function and bile acid metabolism.

Key features

  • Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
  • Broad range of kits across key analytes and targets within the most important metabolic pathways
  • Consistent—strict quality control criteria to ensure reproducible and reliable results
For Research Use Only.
Specifications
Assay Range1.77 to 40 μmol/L
Assay Sensitivity1.36 μmol/L
For Use With (Equipment)Microtiter Plate Reader
Incubation Time3 min.
Interassay CV3.7%
Intraassay CV3.4%
Product TypeKit
Quantity96 Tests
Sample Volume10 μL
Shipping ConditionDry Ice, 2°C to 8°C
Target Kit NamedTotal Bile Acid (TBA)
Total Assay Time20 min.
Detection MethodColorimetric
Immunoassay Kit FormatColorimetric Detection Kit
Sample TypeSerum, Plasma, Animal Tissue
Target SpeciesUniversal
Unit SizeEach
Contents & Storage
Store at 2°C to 8°C.