Thermus thermophilus DNA Ligase

Thermo Scientific™ Thermus thermophilus DNA Ligase is a thermostable NAD-dependent DNA ligase that catalizes formation of phosphodiester bonds between 5`-phospate and 3`-hydroxyl termini in double-stranded DNA. Thermus thermophilus DNA Ligase is not active on RNA, single-stranded DNA, or blunt-ended DNA.

Features

• Increased specificity—high ligation temperature eliminates non-specific ligation
• Stabile at high temperatures with an optimum ligation temperature of 45°C to 65°C

Applications

Thermus thermophilus DNA ligase is recommended for applications requiring high ligation specificity, including:

• Single-stranded nick repair in double-stranded DNA
• Next-generation sequencing
• Rolling circle amplification
• Proximity ligation assay