FluoSpheres™ Carboxylate-Modified Microspheres

Name: FluoSpheres™ Carboxylate-Modified Microspheres
SKU: F10720

Achieve the brightest fluorescence with Carboxylate-Modified FluoSphere Microspheres, available in different colors and particle sizes.

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Catalog Number	Diameter (Metric)	Color	Excitation/Emission	Quantity
F10720	0.04 μm	Yellow-Green, Orange, Red, Dark Red	505/515, 540/560, 580/605, 660/680 nm	1 mL/each
F8803	0.1 μm	Yellow-Green	505/515 nm	10 mL
F8816	1.0 μm	Crimson	625/645 nm	2 mL
F8823	1.0 μm	Yellow-Green	505/515 nm	10 mL
F8801	0.1 μm	Red	580/605 nm	10 mL
F8811	0.2 μm	Yellow-Green	505/515 nm	10 mL
F8807	0.2 μm	Dark Red	660/680 nm	2 mL
F20881	0.2 μm	Orange	365/610 nm	2 mL
F8783 also known as F-8783	0.02 μm	Dark Red	660/680 nm	2 mL
F8786	0.02 μm	Red	580/605 nm	10 mL
F8795 also known as F-8795	0.04 μm	Yellow-Green	505/515 nm	1 mL
F8813	0.5 μm	Yellow-Green	505/515 nm	10 mL
F8820	1.0 μm	Orange	540/560 nm	10 mL
F8825	2.0 μm	Nile Red	535/575 nm	2 mL
F8827	2.0 μm	Yellow-Green	505/515 nm	2 mL
F8781 also known as F-8781	0.02 μm	Blue	365/415 nm	10 mL
F8782 also known as F-8782	0.02 μm	Crimson	625/645 nm	2 mL
F8784	0.02 μm	Nile Red	535/575 nm	10 mL
F8787	0.02 μm	Yellow-Green	505/515 nm	10 mL
F8789 also known as F-8789	0.04 μm	Dark Red	660/680 nm	1 mL
F8792 also known as F-8792	0.04 μm	Orange	540/560 nm	1 mL
F8793	0.04 μm	Red	580/605 nm	1 mL
F8794	0.04 μm	Red-Orange	565/580 nm	1 mL
F8797	0.1 μm	Blue	350/440 nm	10 mL
F8799	0.1 μm	Infrared	715/755 nm	1 mL
F8800 also known as F-8800	0.1 μm	Orange	540/560 nm	10 mL
F8805	0.2 μm	Blue	365/415 nm	10 mL
F8806	0.2 μm	Crimson	625/645 nm	2 mL
F8809	0.2 μm	Orange	540/560 nm	10 mL
F8810	0.2 μm	Red	580/605 nm	10 mL
F8812	0.5 μm	Red	580/605 nm	10 mL
F8814	1.0 μm	Blue	365/415 nm	10 mL
F8815	1.0 μm	Blue	350/440 nm	10 mL
F8819	1.0 μm	Nile Red	535/575 nm	10 mL
F8821	1.0 μm	Red	580/605 nm	10 mL
F8824 also known as F-8824	2.0 μm	Blue	365/415 nm	2 mL
F8826	2.0 μm	Red	580/605 nm	2 mL

37 Options

Catalog number F10720

Price (MXN)

Diameter (Metric):

0.04 μm

Color:

Yellow-Green, Orange, Red, Dark Red

Excitation/Emission:

505/515, 540/560, 580/605, 660/680 nm

Quantity:

1 mL/each

Easily perform flow cytometry, microscopy, HTS, HCS, immunoassay, and other laboratory applications using our extensive selection of FluoSpheres Carboxylate-Modified Microspheres. FluoSphere beads can be used in passive adsorption or active, covalent coupling of proteins, nucleic acids, and biomolecules for particle capture applications. FluoSphere microspheres are loaded with proprietary fluorescent dyes, making them the brightest microspheres available.

Visualize the brightest fluorescence for laboratoy applications including fluorescence microscopy, flow cytometry, HTS, HCS, and cell tracing with our Carboxylate-Modified FluoSphere Microspheres, which are manufactured from polystyrene microspheres and loaded with different proprietary dyes. Using specialized staining methods enables all of the fluorescent dye molecules to be contained inside each polystyrene microsphere instead of on the bead's surface. This protective environment within the bead shields the dye from detrimental environmental effects, such as photobleaching. Our carboxylate-modified microspheres are coated with a hydrophilic polymer containing multiple carboxylic acids for covalent attachment of ligands. A range of particle sizes is available for different research uses and experiments.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Excitation/Emission505/515, 540/560, 580/605, 660/680 nm

Product LineFLUOSPHERES

Quantity1 mL/each

Surface ModificationCarboxylate

ColorYellow-Green, Orange, Red, Dark Red

Diameter (Metric)0.04 μm

For Use With (Application)Fluorescence Microscopy

MaterialPolystyrene

Product TypeCarboxylate-Modified Microsphere

Unit SizeEach

Contents & Storage

Store in refrigerator (2–8°C) and protect from light.

Frequently asked questions (FAQs)

I read that carboxylate-modified latex (CML) beads have a "fluffy coat" of carboxyl groups on their surface. What is meant by this?

The CML beads have a high density of carboxyl groups at the surface. The surface layer is quite hydrophilic and at the appropriate pH (pH>5), are charged; due to electrostatic repulsion, this type of surface is 3-dimensional and may be considered analogous to the fuzz on a tennis ball.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

When does the warranty guarantee expire for FluoSpheres Carboxylated-Modified Microspheres?

FluoSpheres Carboxylate-Modified Microspheres (Cat. Nos. F8783, F8786, F8801, F8807, F8811, F8803, F8816, F8823, F20881, F10720) have a 1-year warranty guarantee, unless otherwise indicated on the Certificate of Analysis (COA).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the warranty for FluoSpheres microspheres?

The warranty period for FluoSpheres microspheres is 1-year from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

Centrifugation is not an effective way to collect smaller microspheres; many particles remain in the solution even if you can visualize a small pellet. For beads less than 1 µm in diameter, we recommend washing by either:

Cross-flow filtration, as these particles have a very high compression modulus and can withstand high g-forces without risk of harm or dialysis with a 500 kDa MWCO
Note: Microspheres greater than 1 µm in diameter can be centrifuged at 1,300 rpm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I've had my microspheres for over a year, and I'm wondering if they're still good to use. What are some good ways to check their functionality?

Bacterial contamination is the most common cause of microspheres becoming unusable. Many of our particles are supplied with a low level of sodium azide to prevent bacterial contamination, but sometimes this can still occur. Bacterial contamination is best assessed by plating on appropriate growth medium and checking the plates after 72 hr.

Find additional tips, troubleshooting help, and resources within ourMicrospheres Support Center.

View all FluoSpheres™ Carboxylate-Modified Microspheres FAQs