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Phusion Blood Direct PCR Master Mix
Phusion Blood Direct PCR Master Mix
Thermo Scientific™

Phusion Blood Direct PCR Master Mix

Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.
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Catalog NumberNo. of Reactions
F175L500 Reactions
F175S100 Reactions
Catalog number F175L
Price (MXN)
-
No. of Reactions:
500 Reactions
Request bulk or custom format
Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion Hot Start II High-Fidelity DNA Polymerase is resistant to main PCR inhibitors present in blood and retains its activity at blood concentrations of up to 40% in the PCR reaction.

This accurate proofreading DNA polymerase makes the Phusion Blood Direct PCR Master Mix an ideal choice for demanding applications, such as cloning. Phusion Blood Direct PCR Master Mix also allows analysis of blood samples with different preservatives (e.g., heparin, EDTA, citrate), storage conditions, and from various species.

Highlights

  • Direct PCR allows sample to be added directly to PCR reaction, reducing time-consuming and expensive DNA purification steps
  • Available Dilution & Storage protocol allows several PCR reactions from one sample
  • Specially engineered Phire Hot Start II DNA polymerase for extremely short PCR protocol times
  • Master mix format with premixed gel loading dye minimizes risk of cross-contamination, reduces sample handling, and allows direct loading on gel

Applications

  • Genotyping
  • Transgene detection
  • Knockout analysis
  • Sequencing
  • Cloning

Notes

  • Annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Hot StartBuilt-In Hot Start
Includes Label or DyeYes
No. of Reactions500 Reactions
OverhangBlunt
PolymerasePhusion Blood II DNA Polymerase
Product TypeDirect PCR Master Mix
Quantity500 reactions
Reaction FormatSuperMix or Master Mix
Sample TypeBlood
Shipping ConditionWet or Dry Ice
Starting MaterialDNA
Concentration2X
For Use With (Application)Genotyping
GC-Rich PCR PerformanceHigh
PCR MethodDirect PCR
Reaction SpeedFast
Unit SizeEach
Contents & Storage
• 2X Phusion Blood Direct PCR Master Mix, 5 x 1 mL
• Universal Control Primer Mix, 40 μL
• 50 mM EDTA, 0.5 mL
• 50 mM DMSO, 0.5 mL
• 50 mM MgCl2, 1.5 mL
• Nuclease-free water, 4 x 1.25 mL
• O'GeneRuler Express DNA Ladder, 50 μg (sufficient for 100 applications)

Store at -20°C.

Frequently asked questions (FAQs)

What is your recommendation to take a sample from plant or animal tissue for Direct PCR?

In order to obtain best results from Direct PCR, it is important to use a very small amount of starting materials. The recommended size is 0.3-0.5 mm in diameter. We recommend using Tissue Puncher 0.3 mm (Cat. No. F-200S), which is a convenient tool for sampling and is suitable for both plant and animal tissues.

Why are Control primers provided in the Direct PCR Kits?

It is very important to include a control reaction in the Direct PCR to ensure that the PCR conditions are optimal for the specific tissue or plant material used. Control primers are universal primers that amplify a small fragment from a conserved genome region. If the reaction with control primers fails, the sample material may not be suitable for Direct PCR, or PCR conditions need optimization.

Can I use plant or tissue sample which is not on the list of the Direct PCR Master Mix and Kit tested samples?

You can test any plant or tissue sample. However, it is recommended to first optimize the reaction. When working with new sample materials, start with the Dilution & Storage protocol as it allows several PCR reactions to be performed from the same sample during optimization. It is recommended to perform a positive control with purified sample DNA to ensure that the PCR conditions are working. If the positive control with purified DNA fails, the PCR conditions should be optimized before continuing further.

Can you amplify the same sample materials with the Direct PCR Master Mixes as with Direct PCR Kits?

Yes, the Direct PCR Master Mixes are upgrades of the Direct PCR kits and thus are suitable for the same sample materials.

What is the highest blood concentration possible in the reaction when using Phusion Blood Direct PCR Master Mix and Kit?

We recommend using blood concentration up to 20%; however, we had successful amplifications with up to 40% of blood in the reaction mix. In some cases optimization may be required to achieve this.

View all Phusion Blood Direct PCR Master Mix FAQs