FluoSpheres™ Size Kit #2, Carboxylate-modified Microspheres, yellow-green fluorescent (505/515), 2% solids, six sizes

Citations & References (38)

Invitrogen™

FluoSpheres™ Size Kit #2, Carboxylate-modified Microspheres, yellow-green fluorescent (505/515), 2% solids, six sizes

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed fromRead more

Catalog Number	Quantity
F8888	1 mL/each

Catalog number F8888

Price (MXN)

Quantity:

1 mL/each

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes™ FluoSpheres™ beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. This FluoSpheres™ Size Kit supplies 1 mL of each the 0.02, 0.1, 0.2, 0.5, 1.0, and 2.0 μm sizes.

FluoSpheres™ Microsphere Specifications

• Label (Ex/Em): Yellow-green fluorescent (505/515)
• Nominal bead diameter: 0.02, 0.1, 0.2, 0.5, 1.0, 2.0 μm
• Coupling surface: Carboxylate
• Solids: 2%

Characteristics of the Various FluoSpheres™ Coupling Surfaces
• Carboxylate-modified FluoSpheres™ beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC.
• Sulfate FluoSpheres™ beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin.
• Aldehyde-sulfate FluoSpheres™ beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres™ beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide.

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres™ Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 μm, 0.04 μm, 0.1 μm, 0.2 μm, 0.5 μm, 1.0 μm, 2.0 μm, 4.0 μm, 10.0 μm, and 15.0 μm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres™ beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 13485:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product LineFLUOSPHERES

Quantity1 mL/each

Shipping ConditionRoom Temperature

Surface ModificationCarboxylate

ColorYellow-Green

Diameter (Metric)0.02, 0.1, 0.2, 0.5, 1, 2 μm

MaterialPolystyrene

Product TypeCarboxylate-Modified Microsphere

Unit SizeEach

Contents & Storage

Store in refrigerator (2–8°C) and protect from light.

Frequently asked questions (FAQs)

What is the warranty for FluoSpheres microspheres?

The warranty period for FluoSpheres microspheres is 1-year from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

Centrifugation is not an effective way to collect smaller microspheres; many particles remain in the solution even if you can visualize a small pellet. For beads less than 1 µm in diameter, we recommend washing by either:

Cross-flow filtration, as these particles have a very high compression modulus and can withstand high g-forces without risk of harm or dialysis with a 500 kDa MWCO
Note: Microspheres greater than 1 µm in diameter can be centrifuged at 1,300 rpm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I've had my microspheres for over a year, and I'm wondering if they're still good to use. What are some good ways to check their functionality?

Bacterial contamination is the most common cause of microspheres becoming unusable. Many of our particles are supplied with a low level of sodium azide to prevent bacterial contamination, but sometimes this can still occur. Bacterial contamination is best assessed by plating on appropriate growth medium and checking the plates after 72 hr.

Find additional tips, troubleshooting help, and resources within ourMicrospheres Support Center.

I accidentally froze my microspheres; can I still use them?

Even brief freezing can cause irreversible aggregation and potential distortion of the bead shape. You should not use these microspheres.

Find additional tips, troubleshooting help, and resources within our Microspheres Support Center.

My protein-coated microspheres appear to be non-specifically binding in my experimental system. Do you have a product that can help reduce these non-specific interactions?

Non-specific binding can often be relieved by a blocking solution, but microspheres seem to require a stronger blocking solution than those most commonly commercially available. Hence, we've developed the BlockAid Blocking Solution (Cat. No. B10710). This reagent is a protein-based blocking solution designed for use with FluoSpheres microspheres and TransFluoSpheres microspheres conjugated to biotin, streptavidin, NeutrAvidin biotin-binding protein, or other proteins. The BlockAid Blocking Solution has proven useful for reducing the nonspecific binding of protein-coated or other macromolecule-coated microspheres in a wide variety of flow cytometry, microscopy, and microarray applications.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all FluoSpheres™ Size Kit #2, Carboxylate-modified Microspheres, yellow-green fluorescent (505/515), 2% solids, six sizes FAQs

Citations & References (38)

Citations & References

Abstract

Altered membrane dynamics of quantum dot-conjugated integrins during osteogenic differentiation of human bone marrow derived progenitor cells.

Authors:Chen H,Titushkin I,Stroscio M,Cho M

Journal:Biophysical journal

PubMed ID:17114225

Functionalized quantum dots offer several advantages for tracking the motion of individual molecules on the cell surface, including selective binding, precise optical identification of cell surface molecules, and detailed examination of the molecular motion without photobleaching. We have used quantum dots conjugated with integrin antibodies and performed studies to quantitatively ... More

Tuftsin binds neuropilin-1 through a sequence similar to that encoded by exon 8 of vascular endothelial growth factor.

Authors:von Wronski MA,Raju N,Pillai R,Bogdan NJ,Marinelli ER,Nanjappan P,Ramalingam K,Arunachalam T,Eaton S,Linder KE,Yan F,Pochon S,Tweedle MF,Nunn AD

Journal:The Journal of biological chemistry

PubMed ID:16371354

Morphology and dynamics of clathrin/GGA1-coated carriers budding from the trans-Golgi network.

Authors:Puertollano R, van der Wel NN, Greene LE, Eisenberg E, Peters PJ, Bonifacino JS

Journal:Mol Biol Cell

PubMed ID:12686608

'Sorting of transmembrane proteins and their ligands at various compartments of the endocytic and secretory pathways is mediated by selective incorporation into clathrin-coated intermediates. Previous morphological and biochemical studies have shown that these clathrin-coated intermediates consist of spherical vesicles with a diameter of 60-100 nm. Herein, we report the use ... More

Transport in lymphatic capillaries. II. Microscopic velocity measurement with fluorescence photobleaching.

Authors:Berk DA, Swartz MA, Leu AJ, Jain RK

Journal:Am J Physiol

PubMed ID:8769769

'Despite its relevance to the physiology of lymph formation and propulsion, the instantaneous flow velocity in single lymphatic capillaries has not been measured to date. The method of fluorescence recovery after photobleaching (FRAP) was adapted for this purpose and used to characterize flow in the lymphatic capillaries in tail skin ... More

Quantitating intracellular transport of polyplexes by spatio-temporal image correlation spectroscopy.

Authors:Kulkarni RP, Wu DD, Davis ME, Fraser SE

Journal:Proc Natl Acad Sci U S A

PubMed ID:15897455

'Quantitatively understanding how nonviral gene delivery vectors (polyplexes) are transported inside cells is essential before they can be optimized for gene therapy and medical applications. In this study, we used spatio-temporal image correlation spectroscopy (ICS) to follow polymer-nucleic acid particles (polyplexes) of various sizes and analyze their diffusive-like and flow ... More

View all FluoSpheres™ Size Kit #2, Carboxylate-modified Microspheres, yellow-green fluorescent (505/515), 2% solids, six sizes citations