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Citations & References (17)
Invitrogen™
HCS CellMask™ Stains
Easily visualize entire cells or individual cell structures during high-content screening (HCS) assays with HCS CellMask stains, which are available in a variety of colors for multiplexing flexibility and can be used immediately after fixation and permeabilization, or after antibody labeling.
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| Catalog Number | Description |
|---|---|
| H32721 | HCS CellMask Deep Red Stain |
| H32720 | HCS CellMask Blue Stain |
| H32714 | HCS CellMask Green Stain |
| H32722 | HCS CellMask Near-IR Stain |
| H32713 | HCS CellMask Orange Stain |
| H32712 | HCS CellMask™ Red Stain |
Catalog number H32721
Price (MXN)
-
Description:
HCS CellMask Deep Red Stain
Identify entire cells (cytoplasm and nucleus) during high-content screening (HCS) assays with the versatile HCS CellMask Red, CellMask Orange, CellMask Green, CellMask Blue, CellMask Deep Red, and CellMask Near-IR stains, which are fluorophores that can be used immediately after fixation and permeabilization, or after antibody labeling. They are available in a variety of colors for multiplexing protocols.
The versatile HCS CellMask Red, CellMask Orange, CellMask Green, CellMask Blue, CellMask Deep Red, and CellMask Near-IR stains are cell delineation tools for high content screening (HCS) platforms, label the entire cell (cytoplasm and nucleus) and provide an accurate backdrop against which the features of interest can be assessed. The HCS CellMask Red and Blue stains replace the HCS CellMask Red cytoplasmic/nuclear (Cat. No. H32711) and HCS CellMask Blue cytoplasmic/nuclear stains (Cat. No. H34558), respectively.
HCS CellMask stains can be applied to cells immediately after fixation or in the last step of multiplexing protocols, and they are compatible with detergent-based cell permeabilization protocols. If only nuclear staining is desired, HCS NuclearMask stains can be used for measuring DNA content in addition to enabling robust cell demarcation. HCS NuclearMask stains are also available in a choice of colors for multiplexing flexibility.
HCS CellMask stains can be applied to cells immediately after fixation or in the last step of multiplexing protocols, and they are compatible with detergent-based cell permeabilization protocols. If only nuclear staining is desired, HCS NuclearMask stains can be used for measuring DNA content in addition to enabling robust cell demarcation. HCS NuclearMask stains are also available in a choice of colors for multiplexing flexibility.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
Specifications
ColorFar-Red, Deep Red
DescriptionHCS CellMask Deep Red Stain
Detection MethodFluorescence
EmissionNear IR
Excitation Wavelength Range650/655
For Use With (Equipment)High Content Instrument
Product LineCellMask
Quantity1 Set
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeStain
SubCellular LocalizationCytoplasm, Cytosol, Nucleus
Unit SizeEach
Contents & Storage
Includes 1 set of vials, providing sufficient material to stain a total of ten 96-well microplates.
Store at ≤-20°C, desiccate, and protect from light.
Store at ≤-20°C, desiccate, and protect from light.
Frequently asked questions (FAQs)
What dyes are used to make the CellMask stains?
Citations & References (17)
Citations & References
Abstract
Matrix architecture dictates three-dimensional migration modes of human macrophages: differential involvement of proteases and podosome-like structures.
Journal:J Immunol
PubMed ID:20018633
'Tissue infiltration of macrophages, although critical for innate immunity, is also involved in pathologies, such as chronic inflammation and cancer. In vivo, macrophages migrate mostly in a constrained three-dimensional (3D) environment. However, in vitro studies, mainly focused on two dimensions, do not provide meaningful clues about the mechanisms involved in
Development of high-content imaging assays for lethal viral pathogens.
Journal:J Biomol Screen
PubMed ID:20639507
'Filoviruses such as Ebola (EBOV) and Marburg (MARV) are single-stranded negative sense RNA viruses that cause acute hemorrhagic fever with high mortality rates. Currently, there are no licensed vaccines or therapeutics to counter filovirus infections in humans. The development of higher throughput/high-content primary screening assays followed by validation using the
Regulation of icam-1 in cells of the monocyte/macrophage system in microgravity.
Journal:
PubMed ID:25654110
Cells of the immune system are highly sensitive to altered gravity, and the monocyte as well as the macrophage function is proven to be impaired under microgravity conditions. In our study, we investigated the surface expression of ICAM-1 protein and expression of ICAM-1 mRNA in cells of the monocyte/macrophage system
Comparison of a high-throughput high-content intracellular Leishmania donovani assay with an axenic amastigote assay.
Journal:Antimicrob Agents Chemother
PubMed ID:23571538
Visceral leishmaniasis is a neglected tropical disease with significant health impact. The current treatments are poor, and there is an urgent need to develop new drugs. Primary screening assays used for drug discovery campaigns have typically used free-living forms of the Leishmania parasite to allow for high-throughput screening. Such screens
IFITM-2 and IFITM-3 but not IFITM-1 restrict Rift Valley fever virus.
Journal:
PubMed ID:23720721
We show that interferon-induced transmembrane protein 1 (IFITM-1), IFITM-2, and IFITM-3 exhibit a broad spectrum of antiviral activity against several members of the Bunyaviridae family, including Rift Valley fever virus (RVFV), La Crosse virus, Andes virus, and Hantaan virus, all of which can cause severe disease in humans and animals.