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Human Spheroid-Qualified Hepatocytes
Gibco™

Human Spheroid-Qualified Hepatocytes

Off-the-shelf cryopreserved human cells for generation of hepatic spheroids in your own lab.
Have Questions?
Catalog NumberQuantity
HMCPSQ1 Vial
Catalog number HMCPSQ
Price (MXN)
59,864.90
Each
Quantity:
1 Vial
Price (MXN)
59,864.90
Each

Gibco Human Spheroid-Qualified Hepatocytes are off-the-shelf cryopreserved human cells for generation of hepatic spheroids in your own lab. Each vial contains a minimum of four million viable cells, sufficient for up to twenty-seven 96-well plates of hepatic spheroids.

  • Maintain cells in culture longer—up to 28 days, >4X longer than cells last in monolayer culture
  • Design your own schedule—cells are ready for plating when you are
  • Interrogate pathways of interest—applicable for toxicity, metabolism, or disease modeling

Simplified protocol

Cryopreserved cells can be thawed and plated at your convenience, in as little as 60 minutes. Try it on your own, or request a demo with an experienced scientist.

Stringent quality control

Our high standards help ensure only the best spheroid-forming lots become available, maximizing your chance of success. In addition, each lot is tested for:

  • Phase I enzyme induction
  • Bile duct formation (CFDA staining)
  • Proper morphology
  • Viability >70%
  • Attachment efficiency
  • Monolayer formation and integrity

Important! For best results, do not attempt hepatic spheroid formation in low attachment plates from other sources. Hepatocyte lots are qualified in Nunclon Sphera plates only and use of other plates can lead to variability in spheroid formation.

Flexible readouts

Analyze single spheroids or pool several for detection in assays such as gene expression, immunostaining, viability, or CYP activity.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Donor SourceSingle Donor
No. of Cells5 Million
Product TypeHepatocytes
Quantity1 Vial
Shipping ConditionDry shipper
Cell LinePrimary
Cell TypeHepatocytes
Culture TypeHepatocyte Cell Culture
FormCryopreserved
SpeciesHuman
TissueLiver
Unit SizeEach
Contents & Storage
>4 million viable cells/vial

Frequently asked questions (FAQs)

Who should I contact if I am looking for hepatocytes with particular characteristics?

Our hepatocytes are prequalified and sold according to application, such as induction, short-term metabolism, and transporter uptake. If you require hepatocytes with particular P450 values or donor specifications, contact our Hepatic Biology Tech Support Team (hepaticproducts@thermofisher.com.com) or (866) 952-3559 to help with specific lot recommendations.

Who can I call if I have technical questions about hepatocyte cell culture?

Thermo Fisher Scientific has a dedicated team to answer questions regarding hepatocyte cell culture. You can send a question by email (hepaticproducts@thermofisher.com) or contact us by phone: US toll-free: (866) 952-3559

Are there guidelines for the procurement of human liver tissue?

Thermofisher Scientific works with a variety of human tissue sources, including Tissue and Organ Procurement Organizations, qualified Research Tissue Organizations and prominent academic and medical centers through collaborations that follow rigorous regulations, certifications and/or accreditations (“Source Facilities”). Tissues obtained through source facilities are consistent with the applicable legal and ethical practices of the United States.

Source Facilities are required by federal law to maintain accurate records and donor confidentiality as well as adhere to certain applicable laws governing tissue used for research globally. These include, but are not limited to:

- 45 CFR Part 46, subparts A, B, C, D where applicable (US federal guidance on the use of human subjects in research)
- Health Insurance Portability and Accountability Act (HIPAA) (addressing issues of confidentiality of personal information)
- National Organ Transplant Act (42 CFR Part 482)
- The Uniform Anatomical Gift Act (UAGA) of 1968, revised 1984

Where do you get your human liver tissue?

The human liver cells are derived from tissue obtained from accredited institutions. Consent is obtained by these institutions from the donor or the donor’s legal next of kin, for the use of the tissue and its derivatives for research purpose.

What ratio of 3D-spheroid hepatocytes to hepatic stellate cells should I use in 3D-co-culture?

We have used a ratio between 10:1 and 3:1 for 3D-spheroid hepatocytes to stellate cells for a successful 3D co-culture. We would recommend testing different ratios as the cells may vary from one type of model to another.

View all Human Spheroid-Qualified Hepatocytes FAQs