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Thermo Scientific™
Luminaris Probe qPCR Master Mix, high ROX
Thermo Scientific Luminaris Color Probe and Luminaris Probe qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-stepRead more
| Catalog Number | No. of Reactions |
|---|---|
| K0934 | 5000 Reactions |
Catalog number K0934
Price (MXN)
-
No. of Reactions:
5000 Reactions
Thermo Scientific Luminaris Color Probe and Luminaris Probe qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template, primers and probe need to be added.
The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1).
The use of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes with ROX at different concentrations as passive reference dye are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).
Highlights
• UDG in the master mix to prevent carry-over contamination• Specificity—Hot Start Taq DNA Polymerase and the optimized buffer reduce primer dimers
• Sensitivitity—single copy detection
• Wide linear range—accurate quantification across 9 orders of magnitude
• Reproducibility and convenience— ready-to-use 2X master mix
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX for different real-time platforms
• Standard cycling protocols
Applications
• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection
Includes
Luminaris Probe High ROX qPCR Master Mix (2X) includes Hot Start Taq Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer. The master mix contains high concentration of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with nuclease-free water.
The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1).
The use of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes with ROX at different concentrations as passive reference dye are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).
Highlights
• UDG in the master mix to prevent carry-over contamination• Specificity—Hot Start Taq DNA Polymerase and the optimized buffer reduce primer dimers
• Sensitivitity—single copy detection
• Wide linear range—accurate quantification across 9 orders of magnitude
• Reproducibility and convenience— ready-to-use 2X master mix
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX for different real-time platforms
• Standard cycling protocols
Applications
• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection
Includes
Luminaris Probe High ROX qPCR Master Mix (2X) includes Hot Start Taq Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer. The master mix contains high concentration of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with nuclease-free water.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Fidelity (vs. Taq)2 X
For Use With (Equipment)7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
No. of Reactions5000 Reactions
PolymeraseTaq DNA Polymerase
Product LineLuminaris
Product TypeqPCR Master Mix
Quantity5,000 reactions
Sample TypeDNA, dsDNA
Sufficient For5000 Reactions
Concentration2X
Detection MethodPrimer-probe
For Use With (Application)Gene Expression
GC-Rich PCR PerformanceHigh
PCR MethodqPCR
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
Contains:
• 4 x 12.5 mL Luminaris Probe High ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG and dNTPs (with dUTP) in an optimized PCR buffer; sufficient for 5000 x 20 μL reactions. The master mix contains high concentration of ROX passive reference dye for specific real-time platforms.
• 2 x 30 mL Nuclease-Free Water
Store at -20°C.
• 4 x 12.5 mL Luminaris Probe High ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG and dNTPs (with dUTP) in an optimized PCR buffer; sufficient for 5000 x 20 μL reactions. The master mix contains high concentration of ROX passive reference dye for specific real-time platforms.
• 2 x 30 mL Nuclease-Free Water
Store at -20°C.