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Invitrogen™
β-Gal Assay Kit
The β-Gal Assay Kit provides the reagents required to quickly measure the levels of active β-galactosidase expressed in cells transfectedRead more
| Catalog Number | Quantity |
|---|---|
| K145501 | 80 mL |
Catalog number K145501
Price (MXN)
-
Quantity:
80 mL
The β-Gal Assay Kit provides the reagents required to quickly measure the levels of active β-galactosidase expressed in cells transfected with plasmids expressing the lacZ gene. lacZ is a bacterial gene often used as a reporter construct in eukaryotic transfection experiments. The gene product of lacZ, β-galactosidase, is resistant to proteolysis in cellular lysates and its activity is easily assayed. β-galactosidase catalyzes the hydrolysis of β-galactosidase such as ortho-nitrophenyl-D-galactopyranoside (ONPG). Hydrolysis of ONPG to the ONP anion produces a bright yellow color with a peak absorbance at 420 nm that can be quantified using a spectrophotometer.
10.7 gene expression
10.7 gene expression
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Assayβ-Gal (lacZ) Assay
Product TypeBeta-Gal Assay Kit
Quantity80 mL
SubstrateONPG
TargetBeta-Galactosidase
Detection MethodColorimetric
Unit SizeEach
Contents & Storage
The β-Gal Assay Kit contains 10X PBS (phosphate buffered saline), lysis buffer, cleavage buffer, β-mercaptoethanol, ONPG, stop buffer. Store ONPG at -20°C. All other components are stored at either room temperature or +4°C. All reagents are guaranteed stable for 6 months when properly stored.