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Citations & References (4)
Invitrogen™
Vivid Colors™ pcDNA™6.2/C-EmGFP-GW/TOPO™ Mammalian Expression Vector
The Vivid Colors™ pcDNA™6.2 Fluorescent Protein TOPO™ Expression Vectors (Figure 1) allow you to rapidly clone your gene and fuseRead more
| Catalog Number | Quantity |
|---|---|
| K35920 | 20 reactions |
Catalog number K35920
Price (MXN)
-
Quantity:
20 reactions
The Vivid Colors™ pcDNA™6.2 Fluorescent Protein TOPO™ Expression Vectors (Figure 1) allow you to rapidly clone your gene and fuse it to the widely used and well characterized Fluorescent Proteins (FPs) from the jellyfish Aequorea victoria (1, 2). These powerful TOPO™ cloning vectors contain the Emerald Green Fluorescent Protein (EmGFP) or the Yellow Fluorescent Protein (YFP) for simple, non-invasive detection of recombinant protein (Figure 2). Both FPs have been humanized for optimal mammalian expression (3). The Vivid Colors™ pcDNA™6.2 Fluorescent Protein TOPO™ Expression Vectors offer:
• Topoisomerase I for one-step, 5-minute TOPO™ cloning of your PCR-amplified gene of interest
• CMV promoter for high-level expression of the recombinant fluorescent fusion protein
• Ability to fuse EmGFP or YFP to the N- or C-terminus of your protein
• Bsd resistance marker for rapid selection of stable cell lines
• Topoisomerase I for one-step, 5-minute TOPO™ cloning of your PCR-amplified gene of interest
• CMV promoter for high-level expression of the recombinant fluorescent fusion protein
• Ability to fuse EmGFP or YFP to the N- or C-terminus of your protein
• Bsd resistance marker for rapid selection of stable cell lines
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Constitutive or Inducible SystemConstitutive
Delivery TypeTransfection
For Use With (Application)Reporter Assays, Subcellular Localization
Product TypeMammalian Expression Vector
Quantity20 reactions
Reporter GeneGFP (EmGFP)
Selection Agent (Eukaryotic)Blasticidin
VectorpcDNA
Cloning MethodTOPO-TA
Product LineGateway, TOPO, Vivid Colors, pcDNA
PromoterCMV
Protein TagGFP (EmGFP)
Unit SizeEach
Contents & Storage
Each pcDNA™6.2-TOPO™ Expression Vector Kit contains two boxes. The TOPO™ box contains linearized and topoisomerase I-activated pcDNA™6.2-TOPO™ vector, control template, primers for sequencing, PCR reaction components, and an expression control plasmid. The TOPO™ box should be stored at -20°C. The One Shot™ box contains transformation reagents including single-use 50 μl aliquots of One Shot™ TOP10 chemically competent E. coli, S.O.C. medium, and pUC19 supercoiled plasmid control. Store competent E. coli at -80°C. All reagents are guaranteed stable for 6 months when properly stored.
Frequently asked questions (FAQs)
Your Gateway-adapted TOPO vectors are supplied with a control template and control primers. Can I obtain the sequence of the control template?
Can GFP fluorescence be detected in cells that have been stained for beta-galactosidase?
What are the recommended filter sets for detection of EmGFP, YFP, CFP, and BFP by fluorescence microscopy?
What are the excitation and emission maxima for your fluorescent proteins (EmGFP, YFP, BFP, CFP, and Cycle 3 GFP)?
Are the fluorescent proteins you offer (EmGFP, YFP, CFP, BFP, and Cycle 3 GFP) humanized?
Citations & References (4)
Citations & References
Abstract
Ultra-high-throughput screening method for the directed evolution of glucose oxidase.
Journal:
PubMed ID:24613019
'Glucose oxidase (GOx) is used in many industrial processes that could benefit from improved versions of the enzyme. Some improvements like higher activity under physiological conditions and thermal stability could be useful for GOx applications in biosensors and biofuel cells. Directed evolution is one of the currently available methods to
Development of a fluorescence-based method for monitoring glucose catabolism and its potential use in a biomass hydrolysis assay.
Journal:Biotechnol Biofuels
PubMed ID:19019221
'The availability and low cost of lignocellulosic biomass has caused tremendous interest in the bioconversion of this feedstock into liquid fuels. One measure of the economic viability of the bioconversion process is the ease with which a particular feedstock is hydrolyzed and fermented. Because monitoring the analytes in hydrolysis and
Lignin depletion enhances the digestibility of cellulose in cultured xylem cells.
Journal:
PubMed ID:23874568
'Plant lignocellulose constitutes an abundant and sustainable source of polysaccharides that can be converted into biofuels. However, the enzymatic digestion of native plant cell walls is inefficient, presenting a considerable barrier to cost-effective biofuel production. In addition to the insolubility of cellulose and hemicellulose, the tight association of lignin with
Surface carbohydrate analysis and bioethanol production of sugarcane bagasse pretreated with the white rot fungus, Ceriporiopsis subvermispora and microwave hydrothermolysis.
Journal:Bioresour Technol
PubMed ID:21903385
'Effects of pretreatments with a white rot fungus, Ceriporiopsis subvermispora, and microwave hydrothermolysis of bagasse on enzymatic saccharification and fermentation were evaluated. The best sugar yield, 44.9 g per 100g of bagasse was obtained by fungal treatments followed by microwave hydrothermolysis at 180°C for 20 min. Fluorescent-labeled carbohydrate-binding modules which